Publication: Comparison of indirect immunofluorescence assays for diagnosis of scrub typhus and murine typhus using venous blood and finger prick filter paper blood spots
Issued Date
2009-05-01
Resource Type
ISSN
00029637
Other identifier(s)
2-s2.0-66149158511
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.80, No.5 (2009), 837-840
Suggested Citation
Rattanaphone Phetsouvanh, Stuart D. Blacksell, Kemajittra Jenjaroen, Nicholas P.J. Day, Paul N. Newton Comparison of indirect immunofluorescence assays for diagnosis of scrub typhus and murine typhus using venous blood and finger prick filter paper blood spots. American Journal of Tropical Medicine and Hygiene. Vol.80, No.5 (2009), 837-840. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27717
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Comparison of indirect immunofluorescence assays for diagnosis of scrub typhus and murine typhus using venous blood and finger prick filter paper blood spots
Other Contributor(s)
Abstract
We performed indirect immunofluorescence assays (IFAs) to compare levels of IgM and IgG antibodies to Orientia tsutsugamushi and Rickettsia typhi in admission-phase serum samples and filter paper blood spots (assayed immediately and stored at 5.4°C and 29°C for 30 days) collected on the same day from 53 adults with suspected scrub typhus and murine typhus admitted to Mahosot Hospital Vientiane, Lao People's Democratic Republic. The sensitivities and specificities of admission-phase filter paper blood spots in comparison to paired sera were between 91% and 95% and 87% and 100%, respectively, for the diagnosis of scrub typhus and murine typhus. The classification of patients as having or not having typhus did not significantly differ after storage of the blood spots for 30 days (P > 0.4) at 5.4°C and 29°C. Because filter paper blood samples do not require sophisticated and expensive storage and transport, they may be an appropriate specimen collection technique for the diagnosis of rickettsial disease in the rural tropics. Copyright © 2009 by The American Society of Tropical Medicine and Hygiene.