Publication: Enhancement of recombinant human BMP-7 bone formation with bmp binding peptide in a rodent femoral defect model
Issued Date
2011-05-01
Resource Type
ISSN
1554527X
07360266
07360266
Other identifier(s)
2-s2.0-79952779064
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Orthopaedic Research. Vol.29, No.5 (2011), 753-759
Suggested Citation
Jen Chung Liao, Shiau Tzu Tzeng, Gun Keorochana, Kwang Bok Lee, Jared S. Johnson, Yuichiro Morishita, Samuel S. Murray, Jeffrey C. Wang Enhancement of recombinant human BMP-7 bone formation with bmp binding peptide in a rodent femoral defect model. Journal of Orthopaedic Research. Vol.29, No.5 (2011), 753-759. doi:10.1002/jor.21252 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12530
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Title
Enhancement of recombinant human BMP-7 bone formation with bmp binding peptide in a rodent femoral defect model
Abstract
Bone morphogenetic binding peptide (BBP) is an 18.5 kDa fragment of a bone matrix protein peptide. A rat femoral defect model was used to test the effect of BBP combined with recombinant human bone morphogenetic protein-7 (rhBMP-7) to induced bone healing. Two doses of BBP (500 and 1000 μg) were tested with two doses of rhBMP-7 (2 and 5 μg), and the results were c ompared with a positive control (10 μg rhBMP-7). Bone healing was evaluated by radiology, manual palpation, microcomputed tomography, and histology. The high dose of 10 μg of rhBMP-7 resulted in a consistent 100% bone union rate and a mature histological appearance on histology, and was used as a positive control. When 1000 μg of BBP was combined with lower doses of BMP-7 (2 μg rhBMP-7 or 5 μg rhBMP-7) significant differences were seen in radiographic scores, manual palpation, and bone volume, when compared to 2 μg rhBMP-7 or 5 μg rhBMP-7 alone. The combination of 1000 μg of BBP and 5 μg rhBMP-7 also achieved 100% fusion rate, induced a larger amount of bone formation, and yielded similar maturity of bone marrow when compared with the high dosage 10 μg rhBMP-7 group. This study demonstrated that when combined together, BBP can enhance the bone healing of rhBMP-7. Improved healing imparted by the addition of BBP may result in lesser amounts of rhBMP-7 needed to achieve union in the clinical setting. Copyright © 2010 Orthopaedic Research Society.