Publication:
17β-Estradiol suppresses MHC class i chain-related B gene expression via an intact GC box

dc.contributor.authorNiramai Sroijaken_US
dc.contributor.authorMathurose Ponglikitmongkolen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-10-19T04:40:17Z
dc.date.available2018-10-19T04:40:17Z
dc.date.issued2013-04-01en_US
dc.description.abstractMajor histocompatibility complex class I chain-related B (MICB) is a membrane-bound glycoprotein involved in both innate and adaptive immunity through its interaction with NKG2D receptors present on γδ T, αβ CD8+ T, and natural killer cells. Factors known to upregulate MICB expression include heat shock, viral or bacterial infection, and tumorigenesis, and here, we explored the effect of 17β-estradiol (E2) on MICB regulation. Physiological concentrations of E2 were found to suppress MICB mRNA and surface protein levels and this effect was antagonized by the antiestrogen ICI 182780. The inhibitory effect of E2 was also observed for other NKG2D ligands, MICA and ULBPs. Evaluation of promoter fragments from the common MICB*00502 allele revealed that inhibition of transcription by E2 required the GC box at-87. The electrophoretic mobility shift assay and supershift analysis established the presence of SP1, SP3, or estrogen receptor α recognition sites within the MICB promoter sequence and interaction of these factors in situ was confirmed by chromatin immunoprecipitation. We conclude that E2 upon forming a complex with its cognate receptor suppresses MICB expression through binding with SP1/SP3 sites within the MICB promoter GC box. These results suggest that the partial benefit of 17β-estradiol on autoimmune diseases may be mediated by reducing the immune NKG2D ligands like MICB. © 2013 Published by NRC Research Press.en_US
dc.identifier.citationBiochemistry and Cell Biology. Vol.91, No.2 (2013), 102-108en_US
dc.identifier.doi10.1139/bcb-2012-0072en_US
dc.identifier.issn12086002en_US
dc.identifier.issn08298211en_US
dc.identifier.other2-s2.0-84875796720en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/31332
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84875796720&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.title17β-Estradiol suppresses MHC class i chain-related B gene expression via an intact GC boxen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84875796720&origin=inwarden_US

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