Publication:
An extended genotyping framework for Salmonella enterica serovar Typhi, the cause of human typhoid

Issued Date

2016-01-01

Resource Type

Article

ISSN

20411723

DOI

10.1038/ncomms12827

Other identifier(s)

2-s2.0-84991011519

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Nature Communications. Vol.7, (2016)

Suggested Citation

Vanessa K. Wong, Stephen Baker, Thomas R. Connor, Derek Pickard, Andrew J. Page, Jayshree Dave, Niamh Murphy, Richard Holliman, Armine Sefton, Michael Millar, Zoe A. Dyson, Gordon Dougan, Kathryn E. Holt, Julian Parkhill, Robert A. Kingsley, Nicholas R. Thomson, Jacqueline A. Keane, James Hadfield, Elizabeth J. Klemm, Simon R. Harris, Amy K. Cain, Samuel Kariuki, Chinyere Okoro, Calman A. MacLennan, Nga Tran Vu Thieu, Duy Pham Thanh, Corinne Thompson, Christiane Dolecek, James I. Campbell, Guy Thwaites, Jeremy Farrar, Paul N. Newton, David Dance, Paul Turner, E. Kim Mulholland, Jane Hawkey, David J. Edwards, Nicholas A. Feasey, François Xavier Weill, Simon Le Hello, Peter J. Hart, Robert F. Breiman, Robert S. Onsare, Conall H. Watson, W. John Edmunds, Melita A. Gordon, Robert S. Heyderman, Chisomo Msefula, Jan Jacobs, Octavie Lunguya, Jose A. Chabalgoity, Mike Kama, Kylie Jenkins, Shanta Dutta, Florian Marks, Josefina Campos, Stephen Obaro, Karen H. Keddy, Anthony M. Smith, Christopher M. Parry, Abhilasha Karkey, Sabina Dongol, Buddha Basnyat, Amit Arjyal, Muriel Dufour, Don Bandaranayake An extended genotyping framework for Salmonella enterica serovar Typhi, the cause of human typhoid. Nature Communications. Vol.7, (2016). doi:10.1038/ncomms12827 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43228

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Title

An extended genotyping framework for Salmonella enterica serovar Typhi, the cause of human typhoid

Author(s)

Vanessa K. Wong
 Stephen Baker
 Thomas R. Connor
 Derek Pickard
 Andrew J. Page
 Jayshree Dave
 Niamh Murphy
 Richard Holliman
 Armine Sefton
 Michael Millar
 Zoe A. Dyson
 Gordon Dougan
 Kathryn E. Holt
 Julian Parkhill
 Robert A. Kingsley
 Nicholas R. Thomson
 Jacqueline A. Keane
 James Hadfield
 Elizabeth J. Klemm
 Simon R. Harris
 Amy K. Cain
 Samuel Kariuki
 Chinyere Okoro
 Calman A. MacLennan
 Nga Tran Vu Thieu
 Duy Pham Thanh
 Corinne Thompson
 Christiane Dolecek
 James I. Campbell
 Guy Thwaites
 Jeremy Farrar
 Paul N. Newton
 David Dance
 Paul Turner
 E. Kim Mulholland
 Jane Hawkey
 David J. Edwards
 Nicholas A. Feasey
 François Xavier Weill
 Simon Le Hello
 Peter J. Hart
 Robert F. Breiman
 Robert S. Onsare
 Conall H. Watson
 W. John Edmunds
 Melita A. Gordon
 Robert S. Heyderman
 Chisomo Msefula
 Jan Jacobs
 Octavie Lunguya
 Jose A. Chabalgoity
 Mike Kama
 Kylie Jenkins
 Shanta Dutta
 Florian Marks
 Josefina Campos
 Stephen Obaro
 Karen H. Keddy
 Anthony M. Smith
 Christopher M. Parry
 Abhilasha Karkey
 Sabina Dongol
 Buddha Basnyat
 Amit Arjyal
 Muriel Dufour
 Don Bandaranayake

Other Contributor(s)

Wellcome Trust Sanger Institute
 Addenbrooke's Hospital
 UCL
 Nuffield Department of Clinical Medicine
 London School of Hygiene & Tropical Medicine
 Cardiff University
 Public Health England
 Barts and The London NHS Trust
 University of Melbourne
 Bio21 Molecular Science and Biotechnology Institute
 Liverpool School of Tropical Medicine
 Quadram Institute Bioscience
 Institut Pasteur, Paris
 University of Birmingham
 Kenya Medical Research Institute
 Centers for Disease Control and Prevention
 Emory Global Health Institute
 University of Liverpool
 University of Malawi College of Medicine
 Prins Leopold Instituut voor Tropische Geneeskunde
 KU Leuven
 National Institute for Biomedical Research
 University Hospital of Kinshasa
 Universidad de la Republica Instituto de Higiene
 Ministry of Health
 null
 National Institute of Cholera and Enteric Diseases India
 International Vaccine Institute, Seoul
 ANLIS-Carlos G Malbran Institute
 University of Nebraska Medical Center
 University of Abuja
 Bingham University
 University of Witwatersrand
 Nagasaki University
 Oxford University Clinical Research Unit
 Murdoch Children's Research Institute
 Institute of Environmental Science and Research Limited (ESR)
 ESR - Kenepuru Science Centre
 Samoa Ministry of Health
 Organisation Mondiale de la Sante
 Hasanuddin University
 Mahosot Hospital
 Tupua Tamasese Meaole Hospital
 Mahidol University
 Chelsea and Westminster Hospital
 University of Otago
 Angkor Hospital for Children
 University of Cambridge
 St Augustine's Hospital

Abstract

© The Author(s) 2016. The population of Salmonella enterica serovar Typhi (S. Typhi), the causative agent of typhoid fever, exhibits limited DNA sequence variation, which complicates efforts to rationally discriminate individual isolates. Here we utilize data from whole-genome sequences (WGS) of nearly 2,000 isolates sourced from over 60 countries to generate a robust genotyping scheme that is phylogenetically informative and compatible with a range of assays. These data show that, with the exception of the rapidly disseminating H58 subclade (now designated genotype 4.3.1), the global S. Typhi population is highly structured and includes dozens of subclades that display geographical restriction. The genotyping approach presented here can be used to interrogate local S. Typhi populations and help identify recent introductions of S. Typhi into new or previously endemic locations, providing information on their likely geographical source. This approach can be used to classify clinical isolates and provides a universal framework for further experimental investigations.

Keyword(s)

Biochemistry, Genetics and Molecular Biology
 Chemistry

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/43228

Collections

Scopus 2016-2017