Publication: ELISA as an alternative tool for epidemiological surveillance for dengue in mosquitoes: A report from Thailand
Issued Date
2007-12-01
Resource Type
ISSN
09729062
Other identifier(s)
2-s2.0-36849073893
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Vector Borne Diseases. Vol.44, No.4 (2007), 272-276
Suggested Citation
Mayuna Srisuphanunt, Ratana Sithiprasasna, Somboon Patpoparn, Watcharee Attatippaholkun, Viroj Wiwanitkit ELISA as an alternative tool for epidemiological surveillance for dengue in mosquitoes: A report from Thailand. Journal of Vector Borne Diseases. Vol.44, No.4 (2007), 272-276. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24483
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Title
ELISA as an alternative tool for epidemiological surveillance for dengue in mosquitoes: A report from Thailand
Abstract
Background & objectives: Dengue fever (DF), dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) are the re-emerging infectious diseases caused by the four serotypes of dengue (DEN) virus, type 1 to 4, belonging to the family Flaviviridae and genus Flavivirus. In the absence of a safe and effective mass immunisation, the prevention and control of dengue outbreaks depend upon the surveillance of cases and mosquito vector. The aim of this work is to test enzyme-linked immunosorbent assay (ELISA) tool for the virological surveillance of dengue. Methods: Virus-infected Aedes mosquitoes were collected from the field in order to serve as an early warning monitoring tool for dengue outbreaks. In a prospective field study conducted from April to September 2000, female adult Aedes mosquitoes were caught from selected dengue-sensitive area in Chombung district, Ratchaburi province and assayed by ELISA. Result: Approximately 18.3% were found positive for dengue virus. Conclusion: This can imply that ELISA can be an alternative tool for epidemiological surveillance for dengue in mosquitoes.