Publication: Lipase-catalyzed synthesis of sucrose monoester: Increased productivity by combining enzyme pretreatment and non-aqueous biphasic medium
Issued Date
2017-10-10
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ISSN
18734863
01681656
01681656
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2-s2.0-85026344609
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Biotechnology. Vol.259, (2017), 182-190
Suggested Citation
Pranee Inprakhon, Natcha Wongthongdee, Taweechai Amornsakchai, Thunyarat Pongtharankul, Panya Sunintaboon, Lars O. Wiemann, Alain Durand, Volker Sieber Lipase-catalyzed synthesis of sucrose monoester: Increased productivity by combining enzyme pretreatment and non-aqueous biphasic medium. Journal of Biotechnology. Vol.259, (2017), 182-190. doi:10.1016/j.jbiotec.2017.07.021 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41747
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Title
Lipase-catalyzed synthesis of sucrose monoester: Increased productivity by combining enzyme pretreatment and non-aqueous biphasic medium
Abstract
© 2017 Elsevier B.V. Sucrose monocaprate was synthesized by carrying out a lipase-catalyzed transesterification in a non-aqueous biphasic medium. Vinyl caprate was mechanically dispersed into a solution of sucrose in DMSO. The use of DMSO allowed increasing sucrose concentration up to 0.7 M (in DMSO). The denaturing effect of DMSO on lipase was avoided by pretreatment of lipase by pH adjustment in the presence of crown ether. This pretreatment maintained a significant catalytic activity which led to 0.2 M sucrose monoester within 1 h at 50 °C, which represented higher productivity than already reported. Detailed structural characterization revealed that only monoester was recovered and the 2-O-acylated sucrose monocaprate was the major isomer in the final product.