Publication: Melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens
Issued Date
2014-01-01
Resource Type
ISSN
18790089
0145305X
0145305X
Other identifier(s)
2-s2.0-84905660372
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Mahidol University
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SCOPUS
Bibliographic Citation
Developmental and Comparative Immunology. Vol.47, No.1 (2014), 150-159
Suggested Citation
Walaiporn Charoensapsri, Piti Amparyup, Chawapat Suriyachan, Anchalee Tassanakajon Melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens. Developmental and Comparative Immunology. Vol.47, No.1 (2014), 150-159. doi:10.1016/j.dci.2014.07.010 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33448
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Title
Melanization reaction products of shrimp display antimicrobial properties against their major bacterial and fungal pathogens
Abstract
Melanization is a rapid defense mechanism in invertebrates. The substrate specificity of phenoloxidases (POs) and the role of melanization reaction products were investigated in the black tiger shrimp, Penaeus monodon. Two PmPOs (PmproPO1 and PmproPO2) were found to display a substrate specificity towards monophenols and diphenols, and exhibit relatively weak activity against 5,6-dihydroxyindole (DHI). Systemic infection of the PmproPO1/2 co-silenced shrimp with the fungus, Fusarium solani, led to a significantly increased mortality, suggesting an important role of PmproPOs in shrimp's defense against fungal infection. Using l-DOPA, dopamine or DHI as a substrate, the melanization reaction products exhibited in vitro antimicrobial activities towards Gram-negative bacteria (Vibrio harveyi and Vibrio parahaemolyticus) and Gram-positive bacteria (Bacillus subtilis), whereas the lower effect was detected against the fungus (F. solani). SEM analysis revealed the morphological changes and damage of cell membranes of V. harveyi and F. solani after treatment with shrimp melanization reaction products. Together, these findings demonstrate the crucial functions of the proPO system and the importance of melanization reaction products in the shrimp's immune defense. © 2014 Elsevier Ltd.
