Publication: Development of a multiplex PCR assay for the identification of eight species members of the Thai Hyrcanus Group (Diptera: Culicidae)
Issued Date
2013-11-01
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ISSN
1347605X
00036862
00036862
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2-s2.0-84886780999
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Mahidol University
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SCOPUS
Bibliographic Citation
Applied Entomology and Zoology. Vol.48, No.4 (2013), 469-476
Suggested Citation
Chayanit Hempolchom, Yasushi Otsuka, Visut Baimai, Sorawat Thongsahuan, Atiporn Saeung, Kritsana Taai, Wichai Srisuka, Pradya Somboon, Wej Choochote Development of a multiplex PCR assay for the identification of eight species members of the Thai Hyrcanus Group (Diptera: Culicidae). Applied Entomology and Zoology. Vol.48, No.4 (2013), 469-476. doi:10.1007/s13355-013-0207-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30960
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Title
Development of a multiplex PCR assay for the identification of eight species members of the Thai Hyrcanus Group (Diptera: Culicidae)
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Abstract
The Hyrcanus Group comprises important vectors of malaria because of Plasmodium vivax Grassi and Feletti and filariasis caused by Brugia malayi Brug in many countries of South, Southeast and East Asian regions. In Thailand, eight species members (Anopheles argyropus Swellengrebel, An. crawfordi Reid, An. nigerrimus Giles, An. nitidus Harrison, Scanlon & Reid, An. paraliae Sandosham, An. peditaeniatus Leicester, An. pursati Laveran and An. sinensis Wiedemann) of the Hyrcanus Group have been recognized. Due to morphological overlap, adult females of the Hyrcanus Group in Thailand have been misidentified markedly among the eight species, particularly when using the traumatic scales of wild-caught specimens from epidemiology and control approaches. Therefore, this study first developed a simple and robust multiplex PCR assay, based on second internal transcribed spacer sequences of ribosomal DNA, for differentiating the eight species members of the Thai Hyrcanus Group. © 2013 The Japanese Society of Applied Entomology and Zoology.