Publication: Lipid and protein oxidation levels in spermatozoa and seminal plasma of Asian Elephants (Elephas maximus) and their relationship with semen parameters
Issued Date
2017-04-01
Resource Type
ISSN
14390531
09366768
09366768
Other identifier(s)
2-s2.0-85008487823
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Mahidol University
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SCOPUS
Bibliographic Citation
Reproduction in Domestic Animals. Vol.52, No.2 (2017), 283-288
Suggested Citation
S. Satitmanwiwat, K. Promthep, K. Buranaamnuay, S. Mahasawangkul, K. Saikhun Lipid and protein oxidation levels in spermatozoa and seminal plasma of Asian Elephants (Elephas maximus) and their relationship with semen parameters. Reproduction in Domestic Animals. Vol.52, No.2 (2017), 283-288. doi:10.1111/rda.12900 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41590
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Title
Lipid and protein oxidation levels in spermatozoa and seminal plasma of Asian Elephants (Elephas maximus) and their relationship with semen parameters
Abstract
© 2017 Blackwell Verlag GmbH Peroxidation damage to spermatozoa and seminal plasma has an important role in sperm quality. Thus, the objective of this study was to determine the levels of lipid and protein oxidation in spermatozoa and seminal plasma of Asian elephants (Elephas maximus) with varying percentage of progressive motility. Lipid and protein oxidation was measured by the thiobarbituric acid-reactive species (TBARS) assay and the 2, 4-dinitrophenylhydrazine (DNPH) carbonyl groups assay, respectively. Fresh semen samples were collected from Asian elephants and classified according to the percentage of motile spermatozoa into good (>60%) and poor (≤20%) motility. Results revealed that seminal plasma malondialdehyde (MDA) and seminal plasma protein carbonyls (PCs) were significantly higher in poor motility than in good motility (p <.05). The MDA and PC levels in seminal plasma were negatively correlated with the percentages of progressive motility (p <.05). In addition, the negative correlation between sperm concentration and seminal plasma MDA level was investigated (p <.05). The sperm viability was also negatively correlated with sperm PC level (p <.05). This study indicated that lipid and protein oxidation has deleterious effect on semen quality of Asian elephants.