Publication: Anti-EpCAM scFv gadolinium chelate: a novel targeted MRI contrast agent for imaging of colorectal cancer
Issued Date
2018-10-01
Resource Type
ISSN
13528661
09685243
09685243
Other identifier(s)
2-s2.0-85046688629
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Mahidol University
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SCOPUS
Bibliographic Citation
Magnetic Resonance Materials in Physics, Biology and Medicine. Vol.31, No.5 (2018), 633-644
Suggested Citation
Kannika Khantasup, Pairash Saiviroonporn, Suwatchai Jarussophon, Warangkana Chantima, Tararaj Dharakul Anti-EpCAM scFv gadolinium chelate: a novel targeted MRI contrast agent for imaging of colorectal cancer. Magnetic Resonance Materials in Physics, Biology and Medicine. Vol.31, No.5 (2018), 633-644. doi:10.1007/s10334-018-0687-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45043
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Title
Anti-EpCAM scFv gadolinium chelate: a novel targeted MRI contrast agent for imaging of colorectal cancer
Abstract
© 2018, ESMRMB. Objectives: The development of targeted contrast agents for magnetic resonance imaging (MRI) facilitates enhanced cancer imaging and more accurate diagnosis. In the present study, a novel contrast agent was developed by conjugating anti-EpCAM humanized scFv with gadolinium chelate to achieve target specificity. Materials and methods: The material design strategy involved site-specific conjugation of the chelating agent to scFv. The scFv monomer was linked to maleimide-DTPA via unpaired cysteine at the scFv C-terminus, followed by chelation with gadolinium (Gd). Successful scFv-DTPA conjugation was achieved at 1:10 molar ratio of scFv to maleimide-DTPA at pH 6.5. The developed anti-EpCAM-Gd-DTPA MRI contrast agent was evaluated for cell targeting ability, in vitro serum stability, cell cytotoxicity, relaxivity, and MR contrast enhancement. Results: A high level of targeting efficacy of anti-EpCAM-Gd-DTPA to an EpCAM-overexpressing HT29 colorectal cell was demonstrated by confocal microscopy. Good stability of the contrast agent was obtained and no cytotoxicity was observed in HT29 cells after 48 h incubation with 25–100 µM of Gd. Favorable imaging was obtained using anti-EpCAM-Gd-DTPA, including 1.8-fold enhanced relaxivity compared with Gd-DTPA, and MR contrast enhancement observed after binding to HT29. Conclusion: The potential benefit of this contrast agent for in vivo MR imaging of colorectal cancer, as well as other EpCAM positive cancers, is suggested and warrants further investigation.