Publication:
Broad and strong memory CD4<sup>+</sup> and CD8<sup>+</sup> T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19

Issued Date

2020-01-01

Resource Type

Article

ISSN

15292916
15292908

DOI

10.1038/s41590-020-0782-6

Other identifier(s)

2-s2.0-85090233862

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Nature Immunology. (2020)

Suggested Citation

Yanchun Peng, Alexander J. Mentzer, Guihai Liu, Xuan Yao, Zixi Yin, Danning Dong, Wanwisa Dejnirattisai, Timothy Rostron, Piyada Supasa, Chang Liu, César López-Camacho, Jose Slon-Campos, Yuguang Zhao, David I. Stuart, Guido C. Paesen, Jonathan M. Grimes, Alfred A. Antson, Oliver W. Bayfield, Dorothy E.D.P. Hawkins, De Sheng Ker, Beibei Wang, Lance Turtle, Krishanthi Subramaniam, Paul Thomson, Ping Zhang, Christina Dold, Jeremy Ratcliff, Peter Simmonds, Thushan de Silva, Paul Sopp, Dannielle Wellington, Ushani Rajapaksa, Yi Ling Chen, Mariolina Salio, Giorgio Napolitani, Wayne Paes, Persephone Borrow, Benedikt M. Kessler, Jeremy W. Fry, Nikolai F. Schwabe, Malcolm G. Semple, J. Kenneth Baillie, Shona C. Moore, Peter J.M. Openshaw, M. Azim Ansari, Susanna Dunachie, Eleanor Barnes, John Frater, Georgina Kerr, Philip Goulder, Teresa Lockett, Robert Levin, Yonghong Zhang, Ronghua Jing, Ling Pei Ho, Eleanor Barnes, Danning Dong, Tao Dong, Susanna Dunachie, John Frater, Philip Goulder, Georgina Kerr, Paul Klenerman, Guihai Liu, Andrew McMichael, Graham Ogg, Yanchun Peng, Mariolina Salio, Xuan Yao, Zixi Yin, J. Kenneth Baillie, Paul Klenerman, Alexander J. Mentzer, Shona C. Moore, Peter J.M. Openshaw, Malcolm G. Semple, David I. Stuart, Richard J. Cornall, Christopher P. Conlon, Paul Klenerman, Gavin R. Screaton, Juthathip Mongkolsapaya, Andrew McMichael, Julian C. Knight, Graham Ogg, Tao Dong Broad and strong memory CD4<sup>+</sup> and CD8<sup>+</sup> T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19. Nature Immunology. (2020). doi:10.1038/s41590-020-0782-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/59134

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Title

Broad and strong memory CD4<sup>+</sup> and CD8<sup>+</sup> T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19

Author(s)

Yanchun Peng
 Alexander J. Mentzer
 Guihai Liu
 Xuan Yao
 Zixi Yin
 Danning Dong
 Wanwisa Dejnirattisai
 Timothy Rostron
 Piyada Supasa
 Chang Liu
 César López-Camacho
 Jose Slon-Campos
 Yuguang Zhao
 David I. Stuart
 Guido C. Paesen
 Jonathan M. Grimes
 Alfred A. Antson
 Oliver W. Bayfield
 Dorothy E.D.P. Hawkins
 De Sheng Ker
 Beibei Wang
 Lance Turtle
 Krishanthi Subramaniam
 Paul Thomson
 Ping Zhang
 Christina Dold
 Jeremy Ratcliff
 Peter Simmonds
 Thushan de Silva
 Paul Sopp
 Dannielle Wellington
 Ushani Rajapaksa
 Yi Ling Chen
 Mariolina Salio
 Giorgio Napolitani
 Wayne Paes
 Persephone Borrow
 Benedikt M. Kessler
 Jeremy W. Fry
 Nikolai F. Schwabe
 Malcolm G. Semple
 J. Kenneth Baillie
 Shona C. Moore
 Peter J.M. Openshaw
 M. Azim Ansari
 Susanna Dunachie
 Eleanor Barnes
 John Frater
 Georgina Kerr
 Philip Goulder
 Teresa Lockett
 Robert Levin
 Yonghong Zhang
 Ronghua Jing
 Ling Pei Ho
 Eleanor Barnes
 Danning Dong
 Tao Dong
 Susanna Dunachie
 John Frater
 Philip Goulder
 Georgina Kerr
 Paul Klenerman
 Guihai Liu
 Andrew McMichael
 Graham Ogg
 Yanchun Peng
 Mariolina Salio
 Xuan Yao
 Zixi Yin
 J. Kenneth Baillie
 Paul Klenerman
 Alexander J. Mentzer
 Shona C. Moore
 Peter J.M. Openshaw
 Malcolm G. Semple
 David I. Stuart
 Richard J. Cornall
 Christopher P. Conlon
 Paul Klenerman
 Gavin R. Screaton
 Juthathip Mongkolsapaya
 Andrew McMichael
 Julian C. Knight
 Graham Ogg
 Tao Dong

Other Contributor(s)

NIHR Oxford Biomedical Research Centre
 Oxford University Hospitals NHS Foundation Trust
 Liverpool University Hospitals NHS Foundation Trust
 Beijing YouAn Hospital, Capital Medical University
 Diamond Light Source
 The Wellcome Centre for Human Genetics
 Worthing Hospital
 The University of Edinburgh
 University of Oxford
 Alder Hey Children's Hospital
 Xinjiang Medical University
 University of Liverpool
 University of York
 Faculty of Medicine, Siriraj Hospital, Mahidol University
 National Heart and Lung Institute
 Nuffield Department of Medicine
 The Sheffield Medical School
 University of Oxford Medical Sciences Division
 ProImmune

Abstract

© 2020, The Author(s), under exclusive licence to Springer Nature America, Inc. The development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines and therapeutics will depend on understanding viral immunity. We studied T cell memory in 42 patients following recovery from COVID-19 (28 with mild disease and 14 with severe disease) and 16 unexposed donors, using interferon-γ-based assays with peptides spanning SARS-CoV-2 except ORF1. The breadth and magnitude of T cell responses were significantly higher in severe as compared with mild cases. Total and spike-specific T cell responses correlated with spike-specific antibody responses. We identified 41 peptides containing CD4+ and/or CD8+ epitopes, including six immunodominant regions. Six optimized CD8+ epitopes were defined, with peptide–MHC pentamer-positive cells displaying the central and effector memory phenotype. In mild cases, higher proportions of SARS-CoV-2-specific CD8+ T cells were observed. The identification of T cell responses associated with milder disease will support an understanding of protective immunity and highlights the potential of including non-spike proteins within future COVID-19 vaccine design.

Keyword(s)

Immunology and Microbiology
 Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/59134

Collections

Scopus 2020