Publication:
DNA barcode identification of freshwater snails in the family Bithyniidae from Thailand.

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tm-ar-jiraporn-2013.pdf (691.53 KB)

Accepted Date

2013-09-19

Issued Date

2013-11-04

Copyright Date

2013

Resource Type

Article

Language

eng

ISSN

1932-6203 (electronic)

DOI

10.1371/journal.pone.0079144

Rights

Mahidol University

Rights Holder(s)

Plos One

Bibliographic Citation

Kulsantiwong J, Prasopdee S, Ruangsittichai J, Ruangjirachuporn W, Boonmars T, Viyanant V, et al. DNA barcode identification of freshwater snails in the family Bithyniidae from Thailand. PLoS One. 2013 Nov 4;8(11):e79144.

Suggested Citation

Jutharat Kulsantiwong, จุฑารัตน์ กุลสันติวงศ์, Sattrachai Prasopdee, ศาสตราชัย ประสพดี, Jiraporn Ruangsittichai, จิราภรณ์ เรืองสิทธิชัย, Wipaporn Ruangjirachuporn, วิภาภรณ์ เรืองจิระชูพร, Thidarut Boonma, ธิดารัตน์ บุญมาศ, Vithoon Viyanan, วิฑูรย์ ไวยนันท์, Pierossi, Paola, Hebert, Paul D. N., Smarn Tesan, สมาน เทศนา DNA barcode identification of freshwater snails in the family Bithyniidae from Thailand.. Kulsantiwong J, Prasopdee S, Ruangsittichai J, Ruangjirachuporn W, Boonmars T, Viyanant V, et al. DNA barcode identification of freshwater snails in the family Bithyniidae from Thailand. PLoS One. 2013 Nov 4;8(11):e79144.. doi:10.1371/journal.pone.0079144 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/754

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Title

DNA barcode identification of freshwater snails in the family Bithyniidae from Thailand.

Author(s)

Jutharat Kulsantiwong
 จุฑารัตน์ กุลสันติวงศ์
 Sattrachai Prasopdee
 ศาสตราชัย ประสพดี
 Jiraporn Ruangsittichai
 จิราภรณ์ เรืองสิทธิชัย
 Wipaporn Ruangjirachuporn
 วิภาภรณ์ เรืองจิระชูพร
 Thidarut Boonma
 ธิดารัตน์ บุญมาศ
 Vithoon Viyanan
 วิฑูรย์ ไวยนันท์
 Pierossi, Paola
 Hebert, Paul D. N.
 Smarn Tesan
 สมาน เทศนา

Corresponding Author(s)

Smarn Tesan

Other Contributor(s)

Mahidol University. Faculty of Tropical Medicine. Department of Medical Entomology

Abstract

Freshwater snails in the family Bithyniidae are the first intermediate host for Southeast Asian liver fluke (Opisthorchis viverrini), the causative agent of opisthorchiasis. Unfortunately, the subtle morphological characters that differentiate species in this group are not easily discerned by non-specialists. This is a serious matter because the identification of bithyniid species is a fundamental prerequisite for better understanding of the epidemiology of this disease. Because DNA barcoding, the analysis of sequence diversity in the 5' region of the mitochondrial COI gene, has shown strong performance in other taxonomic groups, we decided to test its capacity to resolve 10 species/ subspecies of bithyniids from Thailand. Our analysis of 217 specimens indicated that COI sequences delivered species-level identification for 9 of 10 currently recognized species. The mean intraspecific divergence of COI was 2.3% (range 0-9.2 %), whereas sequence divergences between congeneric species averaged 8.7% (range 0-22.2 %). Although our results indicate that DNA barcoding can differentiate species of these medically-important snails, we also detected evidence for the presence of one overlooked species and one possible case of synonymy.

Keyword(s)

Animals
 DNA, Mitochondrial
 Genetic variation
 Open Access article

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/754

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TM-Article