Publication: Role of MAPK and PKA in regulation of rbOCT2-mediated renal organic cation transport
Issued Date
2007-07-01
Resource Type
ISSN
15221466
03636127
03636127
Other identifier(s)
2-s2.0-34548046479
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
American Journal of Physiology - Renal Physiology. Vol.293, No.1 (2007)
Suggested Citation
Sunhapas Soodvilai, Atip Chatsudthipong, Varanuj Chatsudthipong Role of MAPK and PKA in regulation of rbOCT2-mediated renal organic cation transport. American Journal of Physiology - Renal Physiology. Vol.293, No.1 (2007). doi:10.1152/ajprenal.00043.2007 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24168
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Title
Role of MAPK and PKA in regulation of rbOCT2-mediated renal organic cation transport
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Abstract
The effects of protein kinases MAPK and PKA on the regulation of organic cation transporter 2 (OCT2) were investigated both in a heterologous cell system [Chinese hamster ovary (CHO-K1) cells stably transfected with rabbit (rb)OCT2] and in native intact rabbit renal proximal S2 segments. Inhibition of MEK (by U-0126) or PKA (by H-89) reduced transport activity of rbOCT2 in CHO-K1 cells. The inhibitory effect of U-0126 combined with H-89 produced no additive effect, indicating that the action of PKA and MAPK in the regulation of rbOCT2 is in a common pathway. Activation of PKA by forskolin stimulated rbOCT2 activity, and this stimulatory effect was eliminated by H-89, indicating that the stimulation required PKA activation. In S2 segments of rabbit renal proximal tubules, activation of MAPK (by EGF) and PKA (by forskolin) stimulated activity of rbOCT2, and this activation was abolished by U-0126 and H-89, respectively. This is the first study to show that MAPK and PKA are involved, apparently in a common pathway, in the regulation of OCT2 activity in both a heterologous cell system and intact renal proximal tubules. Copyright © 2007 the American Physiological Society.