Publication: Evaluation of mosquito densoviruses for controlling Aedes aegypti (Diptera: Culicidae): Variation in efficiency due to virus strain and geographic origin of mosquitoes
Issued Date
2008-05-01
Resource Type
ISSN
00029637
Other identifier(s)
2-s2.0-45349100247
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.78, No.5 (2008), 784-790
Suggested Citation
Supanee Hirunkanokpun, Jonathan O. Carlson, Pattamaporn Kittayapong Evaluation of mosquito densoviruses for controlling Aedes aegypti (Diptera: Culicidae): Variation in efficiency due to virus strain and geographic origin of mosquitoes. American Journal of Tropical Medicine and Hygiene. Vol.78, No.5 (2008), 784-790. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/19330
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Evaluation of mosquito densoviruses for controlling Aedes aegypti (Diptera: Culicidae): Variation in efficiency due to virus strain and geographic origin of mosquitoes
Other Contributor(s)
Abstract
Four mosquito densovirus strains were assayed for mortality and infectivity against Aedes aegypti larvae from different geographic regions. The viral titers were quantified by real-time PCR using TaqMan technology. First-instar larvae were exposed to the same titer of each densovirus strain for 48 hours. All strains of densoviruses exhibited larvicidal activity and caused more than 80% mortality and infectivity in the three mosquito strains. Aa/DNV-exposed larvae had the highest mortality rate. The mean time to death of Aa/DNV-exposed larvae was shorter than other DNVs-exposed larvae. We can conclude that different densovirus strains exhibit some variations in their pathogenicity to different populations of Ae. aegypti mosquitoes. A few mosquitoes from Chachoengsao and Bangkok exposed to AeDNV and AThDNV survived to the adult stage to lay eggs and showed 22% to 50% vertical transmission in the F1 generation. Phylogenetic analysis of four densovirus strains indicated that mosquito densoviruses are separated into two distinct clades. Copyright © 2008 by The American Society of Tropical Medicine and Hygiene.