Publication: Bottle and biochemical assays on temephos resistance in Aedes aegypti in Thailand
Issued Date
2005-03-01
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-20444437600
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.2 (2005), 417-425
Suggested Citation
Visut Saelim, William G. Brogdon, Jirasak Rojanapremsuk, Saravudh Suvannadabba, Wongdyan Pandii, James W. Jones, Ratana Sithiprasasna Bottle and biochemical assays on temephos resistance in Aedes aegypti in Thailand. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.2 (2005), 417-425. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17060
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Bottle and biochemical assays on temephos resistance in Aedes aegypti in Thailand
Abstract
The bottle bioassay measuring the time-mortality rate is a simplified procedure for detecting insecticide resistance. It can be used with a biochemical microplate assay to identify the mechanism involved. This integrated approach was used to detect temephos resistance in Aedes aegypti from Nonthaburi (lowest use) and Roi Et (highest use). Ae. aegypti BKK1 laboratory strain was used as the susceptible reference strain. The appropriate concentration of insecticide for bottle bioassay was determined empirically for Ae. aegypti BKK1 strain and found to be in the range of 800-1,050 μg/bottle. The time-mortality rate at 800 μg/bottle was 170±8.66 minutes, significantly different from the time-mortality rates in the 850, 900, 950, and 1,050 μg/bottle (p=0.008) concentrations, which were 135±15.00, 140±8.66, 135±15.00, and 125±8.66 minutes, respectively. The cut-off concentration selected for resistance detection was 850μg/bottle. The time-mortality rate for the Roi Et strain was 382±26.41 minutes, significantly higher than the Nonthaburi (150±25.10 minutes) and BKK1 strains (145±20.49 minutes) (p<0.001). The temephos resistance ratio (RR100) for the Ae. aegypti Roi Et strain was 2.64-fold higher at lethal time (LT100) than for the reference Ae. aegypti BKK1 strain. The mean optical density (OD) value from the biochemical microplate assay for the non-specific esterase of the Roi Et strain was higher than the mean OD for the non-specific esterase of both the Nonthaburi and BKK1 strains. Insensitive acetylcholinesterase was not found to be responsible for the resistance in the field-collected mosquitos. This study suggests that esterase detoxification is the primary cause of resistance in the Ae. aegypti population from Roi Et. Both the bottle bioassay and the biochemical microplate assay were proven to be promising tools for initial detection and field surveillance for temephos resistance.