Publication: Diagnosis of typhoid fever by detection of Salmonella typhi antigen in urine
Issued Date
1992-01-01
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ISSN
00951137
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2-s2.0-0026757660
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Clinical Microbiology. Vol.30, No.9 (1992), 2513-2515
Suggested Citation
W. Chaicumpa, Y. Ruangkunaporn, D. Burr, M. Chongsa-Nguan, P. Echeverria Diagnosis of typhoid fever by detection of Salmonella typhi antigen in urine. Journal of Clinical Microbiology. Vol.30, No.9 (1992), 2513-2515. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22297
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Title
Diagnosis of typhoid fever by detection of Salmonella typhi antigen in urine
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Abstract
A monoclonal antibody specific for group D Salmonella antigen 9 was used in an indirect enzyme-linked immunosorbent assay (ELISA) for detecting the antigen in urine specimens collected from patients with clinical typhoid fever in Jakarta, Indonesia. The ELISA had a sensitivity of 95% in identifying patients in whom Salmonella typhi was isolated from hemocultures, 73% in patients in whom S. typhi was isolated from stool specimens, and 40% in patients in whom the organism was isolated from bone marrow cultures. Among patients in whom S. typhi was isolated from blood cultures, the ELISA had a sensitivity of 65% when a single urine specimen was examined and 95% when serially collected urine specimens were examined. A dot blot immunoassay performed on a nitrocellulose filter in parallel had a sensitivity of 85%, versus 83% for the plate ELISA in which S. typhi was isolated from blood, bone marrow, and/or stool specimens. Since S. typhi antigen is intermittently excreted in the urine of patients with typhoid fever, serially collected urine from patients with typhoid should be tested for antigen 9.