Publication: In vitro and in vivo evaluation of amphiphilic chitosan derivatives for inhibition of organic cation transport function
Issued Date
2020-01-01
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ISSN
16629795
10139826
10139826
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2-s2.0-85090361377
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Mahidol University
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SCOPUS
Bibliographic Citation
Key Engineering Materials. Vol.859 KEM, (2020), 45-50
Suggested Citation
Sirima Soodvilai, Sunhapas Soodvila, Warayuth Sajomsang, Theerasak Rojanarata, Prasopchai Patrojanasophon, Praneet Opanasopit In vitro and in vivo evaluation of amphiphilic chitosan derivatives for inhibition of organic cation transport function. Key Engineering Materials. Vol.859 KEM, (2020), 45-50. doi:10.4028/www.scientific.net/KEM.859.45 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/59088
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Title
In vitro and in vivo evaluation of amphiphilic chitosan derivatives for inhibition of organic cation transport function
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Abstract
© 2020 Trans Tech Publications Ltd, Switzerland. This study explored the interaction of amphiphilic chitosan derivatives, N-benzyl-N,O-succinyl chitosan (BSCS), N-naphthyl-N,O-succinyl chitosan (NSCS) and N-octyl-N,O-succinyl chitosan (OSCS), with renal organic cation transporter 2 (OCT2). The influence of amphiphilic chitosan derivatives on renal OCT2 transport function was determined by monitoring the transport of a positively charged substrate into human renal proximal tubular epithelial cells (RPTEC/TERT1 cells), and murine kidney. Amphiphilic chitosan derivatives inhibited 3H-MPP (a substrate of OCT2) transport in the renal cells in a concentration-reliance characteristic. OSCS reduced the accumulation of the cationic drug, cisplatin, in RPTEC/TERT1 cells. This effect was more pronounced than that of other chitosan derivatives. In addition, co-administration of cisplatin and OSCS significantly reduced cisplatin accumulation compared with receiving cisplatin alone. This result was accompanied by the decrease in nephrotoxicity induced by cisplatin. In conclusion, OSCS inhibited OCT2 function and reduced cationic drug disposition in human renal proximal tubular cells and murine kidney.