Publication: The relationship of phosphorylation of membrane proteins with the osmotic fragility and filterability of Plasmodium berghei-infected mouse erythrocytes
Issued Date
1987-07-29
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ISSN
01674889
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2-s2.0-0023667518
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Mahidol University
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SCOPUS
Bibliographic Citation
BBA - Molecular Cell Research. Vol.929, No.3 (1987), 278-287
Suggested Citation
Yongyuth Yuthavong, Temduang Limpaiboon The relationship of phosphorylation of membrane proteins with the osmotic fragility and filterability of Plasmodium berghei-infected mouse erythrocytes. BBA - Molecular Cell Research. Vol.929, No.3 (1987), 278-287. doi:10.1016/0167-4889(87)90254-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15314
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Title
The relationship of phosphorylation of membrane proteins with the osmotic fragility and filterability of Plasmodium berghei-infected mouse erythrocytes
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Abstract
Membrane from Plasmodium berghei-infected mouse erythrocytes showed a pattern of protein phosphorylation which was substantially altered from the normal pattern, with an increase in the phosphorylation of the protein with an apparent molecular weight of 43 000 (M 43), which increased from undetectable in uninfected cells to a maximum in the mature trophozoite stage. Phosphorylation levels of this and other minor bands were strongly correlated with osmotic fragility and filterability. The level of M 43 phosphorylation in membranes from cells which remained intact in a hypotonic medium was 3.82 ± 0.59-times that of lysed cells, compared with the value of 0.76 ± 0.07 calculated from distribution alone. Results found when intact erythrocytes were phosphorylated by incubation with [ 32 P]P i prior to partial lysis were similar to those found when membranes from the lysed and unlysed fractions were subsequently phosphorylated with [γ- 32 P]ATP. Infected erythrocytes which could pass repeatedly through 3-μm polycarbonate filters had a much higher phosphorylation level for the M 43 region than whole infected cells with similar parasitemia and stage distribution. The phosphorylation change could play a role in the control of osmotic and mechanical properties of the infected erythrocytes during maturation. © 1987.