Publication: Paramphistomum cervi: Antigenic profile of adults as recognized by infected cattle sera
Issued Date
2008-02-01
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ISSN
10902449
00144894
00144894
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2-s2.0-37349077215
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Mahidol University
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SCOPUS
Bibliographic Citation
Experimental Parasitology. Vol.118, No.2 (2008), 203-207
Suggested Citation
Panat Anuracpreeda, Chaitip Wanichanon, Prasert Sobhon Paramphistomum cervi: Antigenic profile of adults as recognized by infected cattle sera. Experimental Parasitology. Vol.118, No.2 (2008), 203-207. doi:10.1016/j.exppara.2007.08.005 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/19371
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Title
Paramphistomum cervi: Antigenic profile of adults as recognized by infected cattle sera
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Abstract
An antigenic profile of adult Paramphistomum cervi was revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using sera from cattle naturally infected with P. cervi, Fasciola gigantica and strongylids. SDS-PAGE of whole worm extracts exhibited 26 distinct protein bands. Immunoblotting analysis of these proteins showed five major antigenic bands which were recognized by serum of individual cattle naturally infected with P. cervi. These antigenic proteins had molecular weights ranging from 23 to 116 kDa. One antigenic protein with a molecular weight of 52 kDa exhibited a consistent reaction with sera from all infected cattle. It's diagnostic sensitivity, specificity and accuracy using this test were 100%, 98% and 98.9%, respectively. The positive and negative predictive values were 97.6% and 100%, respectively. This finding suggests that the 52 kDa protein may be a diagnostic antigen for paramphistomosis. © 2007 Elsevier Inc. All rights reserved.