Publication: Caspase-3, a shrimp phosphorylated hemocytic protein is necessary to control YHV infection
Issued Date
2021-07-01
Resource Type
ISSN
10959947
10504648
10504648
Other identifier(s)
2-s2.0-85104417340
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Mahidol University
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SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology. Vol.114, (2021), 36-48
Suggested Citation
Phattara orn Havanapan, Suparat Taengchaiyaphum, Atchara Paemanee, Nuanwan Phungthanom, Sittiruk Roytrakul, Kallaya Sritunyalucksana, Chartchai Krittanai Caspase-3, a shrimp phosphorylated hemocytic protein is necessary to control YHV infection. Fish and Shellfish Immunology. Vol.114, (2021), 36-48. doi:10.1016/j.fsi.2021.04.007 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/75635
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Title
Caspase-3, a shrimp phosphorylated hemocytic protein is necessary to control YHV infection
Abstract
By using immunohistochemistry detection, yellow head virus (YHV) was found to replicate in granule-containing hemocytes including semi-granular hemocytes (SGC) and granular hemocytes (GC) during the early phase (24 h post injection) of YHV-infected shrimp. Higher signal of YHV infection was found in GC more than in SGC. Comparative phosphoproteomic profiles between YHV-infected and non-infected GC reveal a number of phosphoproteins with different expression levels. The phosphoprotein spot with later on identified as caspase-3 in YHV-infected GC is most interesting. Blocking caspase-3 function using a specific inhibitor (Ac-DEVD-CMK) demonstrated high replication of YHV and consequently, high shrimp mortality. The immunohistochemistry results confirmed the high viral load in shrimp that caspase-3 activity was blocked. Caspase-3 is regulated through a variety of posttranslational modifications, including phosphorylation. Analysis of phosphorylation sites of shrimp caspase-3 revealed phosphorylation sites at serine residue. Taken together, caspase-3 is a hemocytic protein isolated from shrimp granular hemocytes with a role in anti-YHV response and regulated through the phosphorylation process.