Publication: Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus
dc.contributor.author | Pitchanee Jariyapong | en_US |
dc.contributor.author | Charoonroj Chotwiwatthanakun | en_US |
dc.contributor.author | Sataporn Direkbusarakom | en_US |
dc.contributor.author | Ikuo Hirono | en_US |
dc.contributor.author | Suwit Wuthisuthimethavee | en_US |
dc.contributor.author | Wattana Weerachatyanukul | en_US |
dc.contributor.other | Walailak University | en_US |
dc.contributor.other | Mahidol University | en_US |
dc.contributor.other | National University Corporation Tokyo University of Marine Science and Technology | en_US |
dc.date.accessioned | 2018-11-23T09:33:24Z | |
dc.date.available | 2018-11-23T09:33:24Z | |
dc.date.issued | 2015-01-01 | en_US |
dc.description.abstract | © 2014 Elsevier B.V. We tested the use of Macrobrachium rosenbergii nodavirus-like particles (MrNv-VLPs) as a delivery mechanism to carry therapeutic agents against white spot syndrome in shrimp. We used constructed double-stranded RNA called VP28 (VP28 dsRNA) against WSSV envelope genes to confer protection against the pathogen. Results showed that MrNv-VLP was able to encapsulate VP28 dsRNA. Using enhanced green fluorescent protein (EGFP) as a reporter, we found that VLP penetrated various shrimp tissues including the muscle, hepatopancreas, and gill. A statistically significant relative survival rate of 44.5% was obtained in the group of shrimp receiving encapsulated VP28 dsRNA-VLP after WSSV challenge as compared to 100% mortality in the control shrimp at 7. days post-infection. Shrimp treated with EGFP dsRNA loaded into MrNv-VLPs showed relatively similar motility rates as those of controls. Moreover, MrNv-VLP encapsulation improved VP28 dsRNA efficiency against WSSV. A higher survival rate of 16.7% was observed in the group of shrimp receiving encapsulated VP28 dsRNA-VLP when compared to those receiving naked VP28 dsRNA. These results indicate that MrNv-VLP is a good candidate for use as a therapeutic delivery system against shrimp diseases due to its self-reassembly property, broad target of various shrimp tissues and immune enhancement. | en_US |
dc.identifier.citation | Aquaculture. Vol.435, (2015), 86-91 | en_US |
dc.identifier.doi | 10.1016/j.aquaculture.2014.09.034 | en_US |
dc.identifier.issn | 00448486 | en_US |
dc.identifier.other | 2-s2.0-84908431508 | en_US |
dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/35240 | |
dc.rights | Mahidol University | en_US |
dc.rights.holder | SCOPUS | en_US |
dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84908431508&origin=inward | en_US |
dc.subject | Agricultural and Biological Sciences | en_US |
dc.title | Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication | |
mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84908431508&origin=inward | en_US |