Publication: Increased smooth muscle actin expression from bone marrow stromal cells under retinoic acid treatment: An attempt for autologous blood vessel tissue engineering
Issued Date
2005-06-01
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ISSN
0125877X
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2-s2.0-27544513826
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.23, No.2-3 (2005), 107-113
Suggested Citation
Aungkura Jerareungrattana, Monnipha Sila-asna, Ahnond Bunyaratvej Increased smooth muscle actin expression from bone marrow stromal cells under retinoic acid treatment: An attempt for autologous blood vessel tissue engineering. Asian Pacific Journal of Allergy and Immunology. Vol.23, No.2-3 (2005), 107-113. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16956
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Title
Increased smooth muscle actin expression from bone marrow stromal cells under retinoic acid treatment: An attempt for autologous blood vessel tissue engineering
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Abstract
Vascular replacement in vital organs is sometimes necessary for human life for example because of atherosclerosis. Blood vessel tissue engineering is applied for autologous transplantations to avoid graft rejections. Stem cells are used for blood vessel tissue engineering because they are the origin of smooth muscle cells, endothelial cells and fibroblasts. This paper shows that bone marrow stromal cells (BMSCs) can be induced to differentiate into the early stage of smooth muscle cells by using 0.01μM retinoic acid. The differentiation of BMSCs to smooth muscle cells was detected by the expression of smooth muscle alpha actin (SM α-actin), the earliest smooth muscle cell marker. The SM α-actin marker expression was demonstrated using indirect immunofluorescence technique and Western blot analysis. The induction of BMSC to form early stages of smooth muscle cells in this study is appropriate for blood vessel tissue engineering because the early stage smooth muscle cells may be stimulated to develop vascular walls with endothelial cells using a co-culture system.