Publication: The expression and distribution of a leptin receptor in the central nervous system, digestive organs, and gonads of the giant freshwater prawn, Macrobrachium rosenbergii
Issued Date
2018-05-01
Resource Type
ISSN
16180372
00651281
00651281
Other identifier(s)
2-s2.0-85045338688
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Acta Histochemica. Vol.120, No.4 (2018), 373-384
Suggested Citation
Jaruwan Poljaroen, Ruchanok Tinikul, Panat Anuracpreeda, Prasert Sobhon, Yotsawan Tinikul The expression and distribution of a leptin receptor in the central nervous system, digestive organs, and gonads of the giant freshwater prawn, Macrobrachium rosenbergii. Acta Histochemica. Vol.120, No.4 (2018), 373-384. doi:10.1016/j.acthis.2018.04.001 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45176
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
The expression and distribution of a leptin receptor in the central nervous system, digestive organs, and gonads of the giant freshwater prawn, Macrobrachium rosenbergii
Other Contributor(s)
Abstract
© 2018 Elsevier GmbH In the present study, the presence and distribution of leptin receptor (LEP-R) in central nervous system, digestive organs, gonads of giant freshwater prawn, Macrobrachium rosenbergii, were investigated with Western blot and immunohistochemistry. By Western blot a LEP-R with a molecular weight (MW) of 100 kDa was detected in the brain, thoracic ganglia, abdominal ganglia, hepatopancreas, all parts of the gastrointestinal tract, ovaries, and testes. In hepatopancreas and foregut, another intense positive band was detected at molecular weight of 30 kDa, which could be an isotype of LEP-R. By immunohistochemistry, LEP-R-ir was detected in the neurons, and neuropils in the brain, thoracic ganglia, and abdominal ganglia. In the gastrointestinal tract, there was intense LEP-R-ir in the apical part of the epithelial cells of the foregut, midgut, and hindgut. In addition, LEP-R-ir was found in the Restzellen(R)cells and Fibrillenzellen(F) cells in the hepatopancreas. In the ovary, LEP-R-ir was detected in early stage of oocytes and mature oocytes. Intense LEP-R-ir was observed in spermatogonia and spermatocytes of the small and orange claw male prawns. In addition, LEP-R was seen in the high epithelium of spermatic ducts from all male morphotypes. In summary, the detection of the LEP-R-ir suggests the existence of a LEP-R in several organs of M. rosenbergii. Through binding with leptin peptide, LEP-R may be an important signaling molecule that has critical functions in modulating and controlling food intake, energy expenditure, and reproduction in this prawn.