Publication: Glutathione-S-transferases from chloroquine-resistant and -sensitive strains of Plasmodium falciparum: What are their differences?
Issued Date
2004-06-01
Resource Type
ISSN
01251562
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2-s2.0-4544337276
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.35, No.2 (2004), 292-299
Suggested Citation
P. Rojpibulstit, S. Kangsadalampai, T. Ratanavalachai, J. Denduangboripant, P. Chavalitshewinkoon-Petmitr Glutathione-S-transferases from chloroquine-resistant and -sensitive strains of Plasmodium falciparum: What are their differences?. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.35, No.2 (2004), 292-299. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21645
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Title
Glutathione-S-transferases from chloroquine-resistant and -sensitive strains of Plasmodium falciparum: What are their differences?
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Abstract
Glutathione-S-transferases (GSTs) from chloroquine-resistant (CQR, K1) and -sensitive (CQS, T9/94) strains of Plasmodium falciparum were studied. The enzymes from both strains exhibited the optimal pH for enzyme catalysis, at pH 7.5, and were stable at temperatures below 60°C. They also showed the highest specific activities toward CDNB and moderate activities to ethacrynic acid (40% of the activity to CDNB) but little or no activity for other substrates. Km and Vmax values for CDNB and GSH, calculated by Lineweaver-Burk plot from both CQR- and CQS-GSTs, were not statistically different (p<0.05). However, the GSTs activity from CQR appeared to be significantly higher than that from CQS. Therefore, we proposed that GSTs from both malarial strains are identical in their functional domain but different in level of gene expression. Furthermore, protein sequence alignment between P. falciparum GST and GSTs from other organisms suggested that the malarial enzyme is closely similar to other GSTs in Sigma, Alpha, Mu and Pi subclasses, probably most to the Alpha group. Characterization of the purified malarial GST in detail would reveal more precise classification and better understanding of its role in malarial detoxification.