Publication: Modeling of Anopheles minimus mosquito NADPH-cytochrome P450 oxidoreductase (CYPOR) and mutagenesis analysis
Issued Date
2013-01-01
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ISSN
14220067
16616596
16616596
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2-s2.0-84878652013
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Molecular Sciences. Vol.14, No.1 (2013), 1788-1801
Suggested Citation
Songklod Sarapusit, Panida Lertkiatmongkol, Panida Duangkaew, Pornpimol Rongnoparut Modeling of Anopheles minimus mosquito NADPH-cytochrome P450 oxidoreductase (CYPOR) and mutagenesis analysis. International Journal of Molecular Sciences. Vol.14, No.1 (2013), 1788-1801. doi:10.3390/ijms14011788 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31421
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Title
Modeling of Anopheles minimus mosquito NADPH-cytochrome P450 oxidoreductase (CYPOR) and mutagenesis analysis
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Abstract
Malaria is one of the most dangerous mosquito-borne diseases in many tropical countries, including Thailand. Studies in a deltamethrin resistant strain of Anopheles minimus mosquito, suggest cytochrome P450 enzymes contribute to the detoxification of pyrethroid insecticides. Purified A. minimus CYPOR enzyme (AnCYPOR), which is the redox partner of cytochrome P450s, loses flavin-adenosine di-nucleotide (FAD) and FLAVIN mono-nucleotide (FMN) cofactors that affect its enzyme activity. Replacement of leucine residues at positions 86 and 219 with phenylalanines in FMN binding domain increases FMN binding, enzyme stability, and cytochrome c reduction activity. Membrane-Bound L86F/L219F-AnCYPOR increases A. minimus P450-mediated pyrethroid metabolism in vitro. In this study, we constructed a comparative model structure of AnCYPOR using a rat CYPOR structure as a template. Overall model structure is similar to rat CYPOR, with some prominent differences. Based on primary sequence and structural analysis of rat and A. minimus CYPOR, C427R, W678A, and W678H mutations were generated together with L86F/L219F resulting in three soluble Δ55 triple mutants. The C427R triple AnCYPOR mutant retained a higher amount of FAD binding and increased cytochrome c reduction activity compared to wild-type and L86F/L219F-Δ55AnCYPOR double mutant. However W678A and W678H mutations did not increase FAD and NAD(P)H bindings. The L86F/L219F double and C427R triple membrane-bound AnCYPOR mutants supported benzyloxyresorufin O-deakylation (BROD) mediated by mosquito CYP6AA3 with a two- to three-fold increase in efficiency over wild-type AnCYPOR. The use of rat CYPOR in place of AnCYPOR most efficiently supported CYP6AA3-mediated BROD compared to all AnCYPORs. © 2013 by the authors; licensee MDPI, Basel, Switzerland.