Publication: A comparison of lysis centrifugation, pour plate, and conventional blood culture methods in the diagnosis of septicaemic melioidosis
Issued Date
1999-01-01
Resource Type
ISSN
00219746
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2-s2.0-0032809083
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Clinical Pathology. Vol.52, No.8 (1999), 616-619
Suggested Citation
Andrew J.H. Simpson, P. A. Howe, V. Wuthiekanun, N. J. White A comparison of lysis centrifugation, pour plate, and conventional blood culture methods in the diagnosis of septicaemic melioidosis. Journal of Clinical Pathology. Vol.52, No.8 (1999), 616-619. doi:10.1136/jcp.52.8.616 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/25757
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Title
A comparison of lysis centrifugation, pour plate, and conventional blood culture methods in the diagnosis of septicaemic melioidosis
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Abstract
Aims - To determine whether quantitative blood culture methods could improve the diagnosis of septicaemic melioidosis. Methods - A comparison of conventional broth based blood cultures, a pour plate method, and a commercial lysis centrifugation (Isolator 10(TM) blood culture system was conducted in 71 Thai patients with severe melioidosis. The time to identification of B pseudomallei was recorded for each method. Results - 42 patients (59%) were septicaemic. Compared with conventional blood culture, the Isolator and pour plate methods had sensitivities of 81% and 61%, respectively. The median times to a positive culture were: Isolator 39.3 hours, pour plates 45.5 hours, broth culture 61.8 hours (p < 0.001 Isolator υ broth). There was a significant inverse correlation between Isolator tube or pour plate quantitative counts and time to detection (r = -0.44 and -0.57, respectively). Mortality was higher in patients who were septicaemic. Conclusions - Routine use of one of these quantitative methods, in addition to conventional broth culture, may lead to earlier diagnosis of septicaemic melioidosis.