Publication: Competition between Burkholderia pseudomallei and B. thailandensis.
Accepted Date
2015-02-19
Issued Date
2015-03-03
Copyright Date
2015
Resource Type
Language
eng
ISSN
1471-2180 (electronic)
Rights
Mahidol University
Rights Holder(s)
BioMed Central
Bibliographic Citation
Ngamdee W, Tandhavanant S, Wikraiphat C, Reamtong O, Wuthiekanun V, Salje J, et al. Competition between Burkholderia pseudomallei and B. thailandensis. BMC Microbiol. 2015 Mar 3;15(1):56.
Suggested Citation
Wikanda Ngamdee, วิกานดา งามดี, Sarunporn Tandhavanant, ศรัณย์พร ตัณฑวนันท์, Chanthiwa Wikraiphat, จันทร์ทิวา วิกรัยพัฒน์, Onrapak Reamtong, อรภัค เรี่ยมทอง, Vanaporn Wuthiekanun, วรรณพร วุฒิเอกอนันต์, Salje, Jeanne, Low, David A, Peacock, Sharon J, Narisara Chantratita, นริศรา จันทราทิตย์ Competition between Burkholderia pseudomallei and B. thailandensis.. Ngamdee W, Tandhavanant S, Wikraiphat C, Reamtong O, Wuthiekanun V, Salje J, et al. Competition between Burkholderia pseudomallei and B. thailandensis. BMC Microbiol. 2015 Mar 3;15(1):56.. doi:10.1186/s12866-015-0395-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/806
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Title
Competition between Burkholderia pseudomallei and B. thailandensis.
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Abstract
BACKGROUND: Burkholderia pseudomallei is a Gram-negative bacterium that causes
melioidosis, an often fatal disease in tropical countries. Burkholderia
thailandensis is a non-virulent but closely related species. Both species are
soil saprophytes but are almost never isolated together.
RESULTS: We identified two mechanisms by which B. pseudomallei affects the growth
of B. thailandensis. First, we found that six different isolates of B.
pseudomallei inhibited the growth of B. thailandensis on LB agar plates. Second,
our results indicated that 55% of isolated strains of B. pseudomallei produced a
secreted compound that inhibited the motility but not the viability of B.
thailandensis. Analysis showed that the active compound was a pH-sensitive and
heat-labile compound, likely a protein, which may affect flagella processing or
facilitate their degradation. Analysis of bacterial sequence types (STs)
demonstrated an association between this and motility inhibition. The active
compound was produced from B. pseudomallei during the stationary growth phase.
CONCLUSION: Taken together, our results indicate that B. pseudomallei inhibits
both the growth and motility of its close relative B. thailandensis. The latter
phenomenon appears to occur via a previously unreported mechanism involving
flagellar processing or degradation.