Publication: TaqMan real-time PCR assay for specific detection of Opisthorchis viverrini DNA in Thai patients with hepatocellular carcinoma and cholangiocarcinoma
14
Issued Date
2008-06-01
Resource Type
ISSN
10902449
00144894
00144894
Other identifier(s)
2-s2.0-43049120574
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Mahidol University
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SCOPUS
Bibliographic Citation
Experimental Parasitology. Vol.119, No.2 (2008), 217-224
Suggested Citation
Nithikoon Suksumek, Kawin Leelawat, Surang Leelawat, Bruce Russell, Usa Lek-Uthai TaqMan real-time PCR assay for specific detection of Opisthorchis viverrini DNA in Thai patients with hepatocellular carcinoma and cholangiocarcinoma. Experimental Parasitology. Vol.119, No.2 (2008), 217-224. doi:10.1016/j.exppara.2008.01.018 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/19325
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Title
TaqMan real-time PCR assay for specific detection of Opisthorchis viverrini DNA in Thai patients with hepatocellular carcinoma and cholangiocarcinoma
Abstract
The aim of this study was to develop TaqMan real-time PCR assay that detected Opisthorchis viverrini DNA from 18 normal and 18 tumor tissue specimens from Thai patients with hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), who underwent liver resection from October 2005 to May 2006. Control liver specimens were seven non-primary liver cancers. A conserved probe representing 100% sequence homology was used as a reference for O. viverrini-specific probe. Five of six tumors (83%) and all six normal tissues from CCA group; and seven of twelve tumors (58%) and ten of twelve normal tissues (83%) from HCC group were found to have O. viverrini DNA. The O. viverrini DNA detection among HCC and CCA patients were not associated (p = 0.193; 90%CI). This RT-PCR will be a useful tool for investigating the relationship between cancer type and presence of the parasite and also for conducting epidemiological surveys. © 2008 Elsevier Inc. All rights reserved.