Publication:
Detection and genetic characterization of norovirus in environmental water samples in Thailand.

dc.contributor.authorLeera Kittigulen_US
dc.contributor.authorApinya Panjangampatthanaen_US
dc.contributor.authorKannika Pombubpaen_US
dc.contributor.authorYuthana Taweekateen_US
dc.contributor.authorSupornwit Pungchittonen_US
dc.contributor.authorPornphan Diraphaten_US
dc.contributor.authorKanokrat Siripanichgonen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-06-11T05:14:30Z
dc.date.available2018-06-11T05:14:30Z
dc.date.issued2012-03-01en_US
dc.description.abstractThe aim of this study was to detect and characterize noroviruses (NoVs) in environmental water samples. One hundred and fourteen water samples were collected from a river and irrigation canals in central Thailand during 2006-2007. NoVs were detected by RT-nested PCR in 13% of the samples. The river samples (22%) contained NoVs at a higher frequency than the irrigation canal samples (4%). Among the 15 NoV-positive samples, 9 harbored genogroup (G) I, 2 samples with GII, and 4 samples with mixed GI and GII. DNA sequencing of PCR amplicons and phylogenetic analysis of partial capsid gene revealed that 5 samples were of genotype GI-2, 1 sample was GI-6, and 1 sample was a mix of GI-2 and GII-unclassified genotypes. NoVs in water samples quantified using quantitative RT-PCR were in the range of 4.91 x 10(2) -1.26 x 10(3) copies/ml for NoV GI and 3.51 x 10(3) copies/ml for NoV GII. This is the first study demonstrating the presence of NoV variants in water samples collected from a river and the adjacent canals of Thailand.en_US
dc.identifier.citationThe Southeast Asian journal of tropical medicine and public health. Vol.43, No.2 (2012), 323-332en_US
dc.identifier.issn01251562en_US
dc.identifier.other2-s2.0-84871917169en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/14900
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84871917169&origin=inwarden_US
dc.subjectMedicineen_US
dc.titleDetection and genetic characterization of norovirus in environmental water samples in Thailand.en_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84871917169&origin=inwarden_US

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