Publication: Detection and genetic characterization of norovirus in environmental water samples in Thailand.
dc.contributor.author | Leera Kittigul | en_US |
dc.contributor.author | Apinya Panjangampatthana | en_US |
dc.contributor.author | Kannika Pombubpa | en_US |
dc.contributor.author | Yuthana Taweekate | en_US |
dc.contributor.author | Supornwit Pungchitton | en_US |
dc.contributor.author | Pornphan Diraphat | en_US |
dc.contributor.author | Kanokrat Siripanichgon | en_US |
dc.contributor.other | Mahidol University | en_US |
dc.date.accessioned | 2018-06-11T05:14:30Z | |
dc.date.available | 2018-06-11T05:14:30Z | |
dc.date.issued | 2012-03-01 | en_US |
dc.description.abstract | The aim of this study was to detect and characterize noroviruses (NoVs) in environmental water samples. One hundred and fourteen water samples were collected from a river and irrigation canals in central Thailand during 2006-2007. NoVs were detected by RT-nested PCR in 13% of the samples. The river samples (22%) contained NoVs at a higher frequency than the irrigation canal samples (4%). Among the 15 NoV-positive samples, 9 harbored genogroup (G) I, 2 samples with GII, and 4 samples with mixed GI and GII. DNA sequencing of PCR amplicons and phylogenetic analysis of partial capsid gene revealed that 5 samples were of genotype GI-2, 1 sample was GI-6, and 1 sample was a mix of GI-2 and GII-unclassified genotypes. NoVs in water samples quantified using quantitative RT-PCR were in the range of 4.91 x 10(2) -1.26 x 10(3) copies/ml for NoV GI and 3.51 x 10(3) copies/ml for NoV GII. This is the first study demonstrating the presence of NoV variants in water samples collected from a river and the adjacent canals of Thailand. | en_US |
dc.identifier.citation | The Southeast Asian journal of tropical medicine and public health. Vol.43, No.2 (2012), 323-332 | en_US |
dc.identifier.issn | 01251562 | en_US |
dc.identifier.other | 2-s2.0-84871917169 | en_US |
dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/14900 | |
dc.rights | Mahidol University | en_US |
dc.rights.holder | SCOPUS | en_US |
dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84871917169&origin=inward | en_US |
dc.subject | Medicine | en_US |
dc.title | Detection and genetic characterization of norovirus in environmental water samples in Thailand. | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication | |
mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84871917169&origin=inward | en_US |