Publication: Iron absorption in hepcidin1 knockout mice
Issued Date
2011-06-14
Resource Type
ISSN
14752662
00071145
00071145
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2-s2.0-79958766886
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Mahidol University
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SCOPUS
Bibliographic Citation
British Journal of Nutrition. Vol.105, No.11 (2011), 1583-1591
Suggested Citation
Patarabutr Masaratana, Abas H. Laftah, Gladys O. Latunde-Dada, Sophie Vaulont, Robert J. Simpson, Andrew T. McKie Iron absorption in hepcidin1 knockout mice. British Journal of Nutrition. Vol.105, No.11 (2011), 1583-1591. doi:10.1017/S0007114510005507 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12469
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Title
Iron absorption in hepcidin1 knockout mice
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Abstract
Hepcidin, the Fe-regulatory peptide, has been shown to inhibit Fe absorption and reticuloendothelial Fe recycling. The present study was conducted to explore the mechanism of in vivo Fe regulation through genetic disruption of hepcidin1 and acute effects of hepcidin treatment in hepcidin1 knockout (Hepc1 -/- ) and heterozygous mice. Hepcidin1 disruption resulted in significantly increased intestinal Fe uptake. Hepcidin injection inhibited Fe absorption in both genotypes, but the effects were more evident in the knockout mice. Hepcidin administration was also associated with decreased membrane localisation of ferroportin in the duodenum, liver and, most significantly, in the spleen of Hepc1 -/- mice. Hypoferraemia was induced in heterozygous mice by hepcidin treatment, but not in Hepc1 -/- mice, 4 h after injection. Interestingly, Fe absorption and serum Fe levels in Hepc1 -/- and heterozygous mice fed a low-Fe diet were not affected by hepcidin injection. The present study demonstrates that hepcidin deficiency causes increased Fe absorption. The effects of hepcidin were abolished by dietary Fe deficiency, indicating that the response to hepcidin may be influenced by dietary Fe level or Fe status. © 2011 The Authors.