Publication: Widely heterogeneous humoral and cellular immunity after mild SARS-CoV-2 infection in a homogeneous population of healthy young men: Heterogenous immunity to SARS-CoV-2
Issued Date
2021-01-01
Resource Type
ISSN
22221751
Other identifier(s)
2-s2.0-85119251667
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Emerging Microbes and Infections. Vol.10, No.1 (2021), 2141-2150
Suggested Citation
Nina Le Bert, Wan Ni Chia, Wei Yee Wan, Alvin Kuo Jing Teo, Samuel Zeng Rong Chong, Nicole Tan, Doreen Soek Chin Tan, Adeline Chia, Iain Beehuat Tan, Kamini Kunasegaran, Qin Xuan Chua, Mohammad Yazid Abdad, Aven Shan Hua Ng, Shawn Vasoo, Julian Xiao Li Ang, Mao Sheng Lee, Louisa Sun, Jinyan Fang, Feng Zhu, Alex R. Cook, Tar Choon Aw, Jingxiang Huang, Clarence Tam, Fong Sin Lee, Hannah Clapham, Enan Jun Kang Goh, Monica Socheata Suor Peou, Shiow Pin Tan, Siew Kim Ong, Lin Fa Wang, Antonio Bertoletti, Li Yang Hsu, Biauw Chi Ong Widely heterogeneous humoral and cellular immunity after mild SARS-CoV-2 infection in a homogeneous population of healthy young men: Heterogenous immunity to SARS-CoV-2. Emerging Microbes and Infections. Vol.10, No.1 (2021), 2141-2150. doi:10.1080/22221751.2021.1999777 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/77339
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Widely heterogeneous humoral and cellular immunity after mild SARS-CoV-2 infection in a homogeneous population of healthy young men: Heterogenous immunity to SARS-CoV-2
Author(s)
Nina Le Bert
Wan Ni Chia
Wei Yee Wan
Alvin Kuo Jing Teo
Samuel Zeng Rong Chong
Nicole Tan
Doreen Soek Chin Tan
Adeline Chia
Iain Beehuat Tan
Kamini Kunasegaran
Qin Xuan Chua
Mohammad Yazid Abdad
Aven Shan Hua Ng
Shawn Vasoo
Julian Xiao Li Ang
Mao Sheng Lee
Louisa Sun
Jinyan Fang
Feng Zhu
Alex R. Cook
Tar Choon Aw
Jingxiang Huang
Clarence Tam
Fong Sin Lee
Hannah Clapham
Enan Jun Kang Goh
Monica Socheata Suor Peou
Shiow Pin Tan
Siew Kim Ong
Lin Fa Wang
Antonio Bertoletti
Li Yang Hsu
Biauw Chi Ong
Wan Ni Chia
Wei Yee Wan
Alvin Kuo Jing Teo
Samuel Zeng Rong Chong
Nicole Tan
Doreen Soek Chin Tan
Adeline Chia
Iain Beehuat Tan
Kamini Kunasegaran
Qin Xuan Chua
Mohammad Yazid Abdad
Aven Shan Hua Ng
Shawn Vasoo
Julian Xiao Li Ang
Mao Sheng Lee
Louisa Sun
Jinyan Fang
Feng Zhu
Alex R. Cook
Tar Choon Aw
Jingxiang Huang
Clarence Tam
Fong Sin Lee
Hannah Clapham
Enan Jun Kang Goh
Monica Socheata Suor Peou
Shiow Pin Tan
Siew Kim Ong
Lin Fa Wang
Antonio Bertoletti
Li Yang Hsu
Biauw Chi Ong
Other Contributor(s)
National Centre for Infectious Diseases
Faculty of Tropical Medicine, Mahidol University
National Cancer Centre, Singapore
A-Star, Genome Institute of Singapore
Singapore General Hospital
National University of Singapore
Nuffield Department of Medicine
Alexandra Hospital, Singapore
Sengkang General Hospital
Faculty of Tropical Medicine, Mahidol University
National Cancer Centre, Singapore
A-Star, Genome Institute of Singapore
Singapore General Hospital
National University of Singapore
Nuffield Department of Medicine
Alexandra Hospital, Singapore
Sengkang General Hospital
Abstract
Background: We studied humoral and cellular responses against SARS-CoV-2 longitudinally in a homogeneous population of healthy young/middle-aged men of South Asian ethnicity with mild COVID-19. Methods: In total, we recruited 994 men (median age: 34 years) post-COVID-19 diagnosis. Repeated cross-sectional surveys were conducted between May 2020 and January 2021 at six time points–day 28 (n = 327), day 80 (n = 202), day 105 (n = 294), day 140 (n = 172), day 180 (n = 758), and day 280 (n = 311). Three commercial assays were used to detect anti-nucleoprotein (NP) and neutralizing antibodies. T cell response specific for Spike, Membrane and NP SARS-CoV-2 proteins was tested in 85 patients at day 105, 180, and 280. Results: All serological tests displayed different kinetics of progressive antibody reduction while the frequency of T cells specific for different structural SARS-CoV-2 proteins was stable over time. Both showed a marked heterogeneity of magnitude among the studied cohort. Comparatively, cellular responses lasted longer than humoral responses and were still detectable nine months after infection in the individuals who lost antibody detection. Correlation between T cell frequencies and all antibodies was lost over time. Conclusion: Humoral and cellular immunity against SARS-CoV-2 is induced with differing kinetics of persistence in those with mild disease. The magnitude of T cells and antibodies is highly heterogeneous in a homogeneous study population. These observations have implications for COVID-19 surveillance, vaccination strategies, and post-pandemic planning.