Partial extraction method for the rapid analysis of total lipids and γ-oryzanol contents in rice bran

Abstract

Total lipids and γ-oryzanol in rice bran were determined by a partial extraction method. The results agreed well with the conventional total extraction methods. The proposed method uses fewer hazardous organic solvents, takes a shorter extraction time and requires no special extraction apparatus. Total lipids and γ-oryzanol in nine rice bran varieties were analysed by the developed technique. Daw Dum 5647 had the highest total lipids and γ-oryzanol while the lowest content was found in KD XBT 313-19-1-1 and SP XBT 43-7, respectively. The adsorption coefficient (Kd) of the lipids and γ-oryzanol, between hexane and bran, at 30 °C are between 1.16 and 2.00 and 2.02 and 2.65, respectively (depending on the moisture content of the bran). From the Kdvalues, it was estimated that about 92-95% of the lipids and 95-96% of the γ-oryzanol were extracted into hexane at a 10:1 (v/w) ratio of hexane to bran. The effect of solvents on the extraction of γ-oryzanol from rice bran was also studied. It was found that isopropanol was the most suitable solvent for extraction and determination of γ-oryzanol in rice bran. It showed better agreement with the total extraction method. © 2007 Elsevier Ltd. All rights reserved.