Publication: Photocleavage of avidin by a new pyrenyl probe
Issued Date
2011-06-02
Resource Type
ISSN
18732682
10111344
10111344
Other identifier(s)
2-s2.0-79955937531
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Photochemistry and Photobiology B: Biology. Vol.103, No.3 (2011), 251-255
Suggested Citation
Pinpinat Malaikaew, Jisnuson Svasti, Challa V. Kumar, Apinya Buranaprapuk Photocleavage of avidin by a new pyrenyl probe. Journal of Photochemistry and Photobiology B: Biology. Vol.103, No.3 (2011), 251-255. doi:10.1016/j.jphotobiol.2011.04.003 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/11533
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Photocleavage of avidin by a new pyrenyl probe
Other Contributor(s)
Abstract
In this study, a new small-molecule-based reagent was designed to recognize and bind to specific site in protein. A new pyrenyl probe, d-biotinyl-1(1- pyrene)methylamide (Py-biotin) was designed and synthesized by coupling of d-biotin to 1(1-pyrene)methylamine hydrochloride. Binding studies and site-specific photocleavage of avidin by Py-biotin were demonstrated. Binding of Py-biotin to avidin was studied using absorbance and fluorescence spectroscopic techniques. Red shifts of the absorption peak positions of the pyrenyl chromophore followed by hyperchromism were observed upon binding to avidin. The photocleavage of avidin was achieved when a mixture of the protein, Py-biotin, and an electron acceptor, cobalt(III) hexammine trichloride (CoHA), was irradiated at 342 nm. No reaction occurred in the absence of the probe, CoHA, or light. N-terminal sequencing of the peptide fragments indicated a cleavage site of avidin between Thr 77 and Val 78. The high specificity of photocleavage may be valuable in targeting specific sites of proteins with small molecules. © 2011 Elsevier B.V. All rights reserved.