Publication: 16S ribosomal RNA gene-based phylogenetic analysis of abundant bacteria in river, canal and potable water in Bangkok, Thailand
Issued Date
2013-05-01
Resource Type
ISSN
13475215
09186158
09186158
Other identifier(s)
2-s2.0-84876916722
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biological and Pharmaceutical Bulletin. Vol.36, No.5 (2013), 872-876
Suggested Citation
Nobuyasu Yamaguchi, Takahiro Nishiguchi, Fuangfa Utrarachkij, Orasa Suthienkul, Masao Nasu 16S ribosomal RNA gene-based phylogenetic analysis of abundant bacteria in river, canal and potable water in Bangkok, Thailand. Biological and Pharmaceutical Bulletin. Vol.36, No.5 (2013), 872-876. doi:10.1248/bpb.b13-00012 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/32741
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
16S ribosomal RNA gene-based phylogenetic analysis of abundant bacteria in river, canal and potable water in Bangkok, Thailand
Other Contributor(s)
Abstract
In Southeast Asian countries, industrialization and urbanization is occurring rapidly, and water pollution in rivers and canals poses serious problems in some areas, especially in cities. Excess inflow of domestic, agricultural, and industrial wastewater to freshwater environments disturbs the aquatic microbial ecosystem, which can further pollute water by inhibiting biodegradation of pollutants. Therefore, monitoring of microbes in freshwater environment is important to identify changes in indigenous microbial populations and to estimate the influence of wastewater inflows on them. Polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) analysis is suitable for monitoring changes in microbial communities caused by human activities, but this method can be difficult in eutrophic freshwater samples that contain PCR inhibitors. In this study, we optimized DNA extraction procedures and PCR conditions for DGGE analysis of bacterial populations in freshwater samples (canal, river, and tap water) collected in Bangkok, Thailand. A simple freeze-thaw procedure was effective for extracting DNA from bacterial cells in the samples, and LA Taq with added bovine serum albumin provided the best PCR amplification. The PCR-DGGE approach revealed that the most common bacteria in freshwater samples belonged to Gammaproteobacteria, while a Gram-positive bacterium was present at Bangkok Noi Canal. Temporally and spatially continuous analyses of bacterial populations in Bangkok canals and rivers by PCR-DGGE approach should be useful to recognize disturbances of microbial ecosystems caused by excess inflows of wastewater. © 2013 The Pharmaceutical Society of Japan.