Publication: Comparison of point-of-care test and enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies in the diagnosis of human schistosomiasis japonica
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Issued Date
2021-06-01
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ISSN
18783511
12019712
12019712
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2-s2.0-85105454608
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Infectious Diseases. Vol.107, (2021), 47-52
Suggested Citation
Rutchanee Rodpai, Lakkhana Sadaow, Patcharaporn Boonroumkaew, Weeraya Phupiewkham, Tongjit Thanchomnang, Yanin Limpanont, Phiraphol Chusongsang, Oranuch Sanpool, Hiroshi Ohmae, Hiroshi Yamasaki, Pewpan M. Intapan, Wanchai Maleewong Comparison of point-of-care test and enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies in the diagnosis of human schistosomiasis japonica. International Journal of Infectious Diseases. Vol.107, (2021), 47-52. doi:10.1016/j.ijid.2021.04.039 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/78172
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Title
Comparison of point-of-care test and enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies in the diagnosis of human schistosomiasis japonica
Abstract
Objective: Schistosomiasis japonica is an important helminthic disease in Asia. Sensitive and accurate diagnostic tools are indispensable for clinical diagnosis, screening infection and monitoring its control. In this study, we developed an immunochromatographic test (Sj-ICT) to detect anti-Schistosoma japonicum immunoglobulin G antibodies in human sera. Methods: Somatic extract from adult S. japonicum was used as an antigen. The Sj-ICT was developed and optimized as a point-of-care test. All 214 human serum samples were evaluated for diagnostic usefulness and comparison with an enzyme-linked immunosorbent assay (ELISA). Results: The diagnostic sensitivity, specificity, positive and negative predictive values, and accuracy of the Sj-ICT were 90.8%, 87.9%, 86.4%, 91.9% and 89.3%, respectively. For ELISA the values were respectively 91.8%, 87.9%, 86.5%, 92.7% and 89.7%. The concordance between both methods was 86.4 % (Cohen's kappa value = 0.729). Conclusions: The immunochromatographic test kit developed can support clinical diagnosis and large-scale surveys in endemic areas without requiring additional facilities or ancillary supplies.