Publication: Evaluation of recombinant serine protease inhibitor from trichinella spiralis for immunodiagnosis of swine trichinosis
Issued Date
2012-11-05
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ISSN
01251562
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2-s2.0-84868124859
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.43, No.5 (2012), 1094-1104
Suggested Citation
Supaporn Nuamtanong, Paron Dekumyoy, Poom Adisakwattana Evaluation of recombinant serine protease inhibitor from trichinella spiralis for immunodiagnosis of swine trichinosis. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.43, No.5 (2012), 1094-1104. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/14525
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Title
Evaluation of recombinant serine protease inhibitor from trichinella spiralis for immunodiagnosis of swine trichinosis
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Abstract
Serine protease inhibitors, known as serpins, are mainly expressed in newborn and early-stage Trichinella spiralis larvae, suggesting that T. spiralis serpin (TsSERP) could be used as antigen for the immunodiagnosis of swine trichinosis. We produced His-tagged recombinant TsSERP (rTsSERP) in Escherichia coli and purified it using a Co 2+ -affinity column. Western blot (WB) and enzyme-linked immunosorbent assay (ELISA) were performed to determine T. spiralis-infected swine sera samples (n = 5), negative controls (n = 26), and other parasite-infected samples (n = 83). WB showed that T. spiralis-infected sera initially reacted with rTsSERP at day 6 post-infection (dpi), and more strongly in late infection (62 and 84 dpi). However, other parasite-infected sera also elicited cross-reactivity to rTsSERP. On the other hand, indirect ELISA showed that TsSERP was an appropriate antigen for detecting late ( > 60 dpi) but not early infection. No cross-reaction was observed with other parasite-infected sera. Sensitivity and specificity of TsSERP-ELISA at 62 dpi was 80% and 100%, respectively, and at 84 dpi 100% and 100%, respectively. These preliminary results show that TsSERP-ELISA method is suitable for the diagnosis of swine trichinosis, and could become the standard test for diagnosis of trichinosis in several hosts, including humans.