Publication: BCR/ABL rearrangement in philadelphia chromosome negative CML patients
Issued Date
2000-12-01
Resource Type
ISSN
01252208
Other identifier(s)
2-s2.0-0034151837
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.83, No.SUPPL. 1 (2000)
Suggested Citation
Budsaba Rerkamnuaychoke BCR/ABL rearrangement in philadelphia chromosome negative CML patients. Journal of the Medical Association of Thailand. Vol.83, No.SUPPL. 1 (2000). Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/26065
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Title
BCR/ABL rearrangement in philadelphia chromosome negative CML patients
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Abstract
Reverse transcription-polymerase chain reaction (RT-PCR) was used to study 34 patients with chronic myelogenous leukemia (CML) associated with negative Philadelphia (Ph) chromosome. This report showed evidence of a chimeric BCR/ABL transcript in 18 (52.9%) and 28 (82.4%) cases by first PCR and seminested PCR, respectively. In these BCR/ABL transcript positive cases, the incidence of BCR exon3/ABL exon2 (B3A2) and BCR exon 2/ABL exon2 rearrangement was 25 (89.3%) and 3 (10.7%) cases, respectively. The other 6 Ph negative patients showed no evidence of reciprocal translocation of BCR to chromosome 9. This data demonstrates that seminested PCR is sufficiently sensitive to detect BCR/ABL fusion transcript in Ph chromosome negative CML patients.