Publication: Selection of Burkholderia pseudomallei protease-binding peptides by phage display
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Issued Date
2000-11-20
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ISSN
01415492
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2-s2.0-0033773994
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Mahidol University
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SCOPUS
Bibliographic Citation
Biotechnology Letters. Vol.22, No.20 (2000), 1597-1602
Suggested Citation
Wimol Petkanchanapong, Sarah Fredriksson, Virapong Prachayasittikul, Leif Bülow Selection of Burkholderia pseudomallei protease-binding peptides by phage display. Biotechnology Letters. Vol.22, No.20 (2000), 1597-1602. doi:10.1023/A:1005652303282 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/25846
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Title
Selection of Burkholderia pseudomallei protease-binding peptides by phage display
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Abstract
Burkholderia pseudomallei is a causative agent of melioidosis, a fatal community acquired septicemia in Southeast Asia and Northern Australia. A protease has been proposed to be one of the major pathogenic factors to play a significant role in melioidosis. We have used phage display technology to identify peptides binding to B. pseudomallei protease. By screening a constrained cyclic heptapeptide library, five independent clones with affinity to this protease were isolated and the amino acid sequences were determined. The cyclic heptapeptides from two of the phage clones (Cys-Phe-Phe-Met-Pro-His-Thr-Phe-Cys) were identical and showed the strongest phage-protease interaction as detected by ELISA. Four of the five selected phages at the amount of 1013phages could inhibit B. pseudomallei protease activity by approximately 50%.