Publication: Enzyme-linked immunosorbent assay using fragment antigen-binding (Fab) antibody for determination of deoxymiroestrol, a potent phytoestrogen from Pueraria candollei
Issued Date
2021-01-01
Resource Type
ISSN
14653443
09540105
09540105
Other identifier(s)
2-s2.0-85109139597
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Food and Agricultural Immunology. Vol.32, No.1 (2021), 336-348
Suggested Citation
Worapol Sae-Foo, Supaluk Krittanai, Wipawee Juengsanguanpornsuk, Gorawit Yusakul, Tharita Kitisripanya, Seiichi Sakamoto, Waraporn Putalun Enzyme-linked immunosorbent assay using fragment antigen-binding (Fab) antibody for determination of deoxymiroestrol, a potent phytoestrogen from Pueraria candollei. Food and Agricultural Immunology. Vol.32, No.1 (2021), 336-348. doi:10.1080/09540105.2021.1946486 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/75781
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Enzyme-linked immunosorbent assay using fragment antigen-binding (Fab) antibody for determination of deoxymiroestrol, a potent phytoestrogen from Pueraria candollei
Other Contributor(s)
Abstract
Deoxymiroestrol is the most potent phytoestrogen in the chromene group found in Pueraria candollei. The estrogenic activity of P. candollei, functions as hormone replacement therapy in postmenopausal women. This study established an enzyme-linked immunosorbent assay (ELISA) for quantifying deoxymiroestrol using fragment antigen-binding (Fab) antibody to improve analytical performance. Our ELISA specifically detected deoxymiroestrol in the range of 31.25–1,000 ng/mL. Intra- and inter-assay precision was 1.48%–7.11% and 0.58%–9.31%, respectively, the recovery of authentic deoxymiroestrol spiked into samples was 99.77%–101.61%, and the limit of detection was 30.80 ng/mL. The findings of P. candollei root bark determined using our developed ELISA with Fab antibody and an ELISA with anti-deoxymiroestrol polyclonal antibody were consistent (R2 = 0.9807). The developed ELISA can be applied to the quality control of deoxymiroestrol derived from P. candollei.