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Novel virus discovery in field-collected mosquito larvae using an improved system for rapid determination of viral RNA sequences (RDV ver4.0)

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dc.contributor.authorTakuya Yamaoen_US
dc.contributor.authorYuki Eshitaen_US
dc.contributor.authorYuki Kiharaen_US
dc.contributor.authorTomomitsu Sathoen_US
dc.contributor.authorMakoto Kurodaen_US
dc.contributor.authorTsuyoshi Sekizukaen_US
dc.contributor.authorMiho Nishimuraen_US
dc.contributor.authorKouji Sakaien_US
dc.contributor.authorShumpei Watanabeen_US
dc.contributor.authorHiroomi Akashien_US
dc.contributor.authorYupha Rongsriyamen_US
dc.contributor.authorNarumon Komalamisraen_US
dc.contributor.authorRaweewan Srisawaten_US
dc.contributor.authorTakeshi Miyataen_US
dc.contributor.authorAkira Sakataen_US
dc.contributor.authorMasato Hosokawaen_US
dc.contributor.authorManabu Nakashimaen_US
dc.contributor.authorNobuhiro Kashigeen_US
dc.contributor.authorFumio Miakeen_US
dc.contributor.authorShuetsu Fukushien_US
dc.contributor.authorMina Nakauchien_US
dc.contributor.authorMasayuki Saijoen_US
dc.contributor.authorIchiro Kuraneen_US
dc.contributor.authorShigeru Morikawaen_US
dc.contributor.authorTetsuya Mizutanien_US
dc.contributor.otherFukuoka Universityen_US
dc.contributor.otherOita Universityen_US
dc.contributor.otherNational Institute of Infectious Diseasesen_US
dc.contributor.otherGraduate School of Agricultural and Life Sciences The University of Tokyoen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-09-13T06:44:53Z
dc.date.available2018-09-13T06:44:53Z
dc.date.issued2009-01-01en_US
dc.description.abstractIn this study, we improved a method for rapid determination of viral RNA sequences (RDV) to overcome the limitations of previous versions. The RDV ver4.0 method can detect RNA sequences with at least 1,000 copies as starting material. A novel virus, which was isolated from field-collected Aedes aegypti larvae in the Phasi Charoen district of Thailand using C6/36 cells, was identified using the RDV ver4.0 protocol. The virus was named Phasi Charoen virus (PhaV). We used a high-throughput pyrosequencing approach to obtain more information about the genome sequence of PhaV. Analysis of a phylogenic tree based on amino acid sequences strongly suggested that PhaV belongs to the family Bunyaviridae. © 2008 Springer-Verlag.en_US
dc.identifier.citationArchives of Virology. Vol.154, No.1 (2009), 153-158en_US
dc.identifier.doi10.1007/s00705-008-0285-5en_US
dc.identifier.issn03048608en_US
dc.identifier.other2-s2.0-58149326741en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/27747
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=58149326741&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.titleNovel virus discovery in field-collected mosquito larvae using an improved system for rapid determination of viral RNA sequences (RDV ver4.0)en_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=58149326741&origin=inwarden_US

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