Publication: Molecular differentiation of Schistosoma japonicum and Schistosoma mekongi by real-time PCR with high resolution melting analysis
Issued Date
2013-12-01
Resource Type
ISSN
17380006
00234001
00234001
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2-s2.0-84893299515
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Mahidol University
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SCOPUS
Bibliographic Citation
Korean Journal of Parasitology. Vol.51, No.6 (2013), 651-656
Suggested Citation
Amornmas Kongklieng, Worasak Kaewkong, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Viraphong Lulitanond, Pusadee Sri-Aroon, Yanin Limpanont, Wanchai Maleewong Molecular differentiation of Schistosoma japonicum and Schistosoma mekongi by real-time PCR with high resolution melting analysis. Korean Journal of Parasitology. Vol.51, No.6 (2013), 651-656. doi:10.3347/kjp.2013.51.6.651 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31827
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Title
Molecular differentiation of Schistosoma japonicum and Schistosoma mekongi by real-time PCR with high resolution melting analysis
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Abstract
Human schistosomiasis caused by Schistosoma japonicum and Schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of Asia. Due to morphological similarities of cercariae and eggs of these 2 species, microscopic differentiation is difficult. High resolution melting (HRM) real-time PCR is developed as an alternative tool for the detection and differentiation of these 2 species. A primer pair was designed for targeting the 18S ribosomal RNA gene to generate PCR products of 156 base pairs for both species. The melting points of S. japonicum and S. mekongi PCR products were 84.5±0.07 °C and 85.7±0.07 °C, respectively. The method permits amplification from a single cercaria or an egg. The HRM real-time PCR is a rapid and simple tool for differentiation of S. japonicum and S. mekongi in the intermediate and final hosts. © 2013, Korean Society for Parasitology and Tropical Medicine.