Publication: Prevalence of HIV-1 polymerase gene mutations in pre-treated patients in Thailand.
Issued Date
2002-03-01
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-0036490823
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
The Southeast Asian journal of tropical medicine and public health. Vol.33, No.1 (2002)
Suggested Citation
Wasun Chantratita, Ekachai Jenwitheesuk, Chotip Watitpun, Viroj Pongthanapisith, Asda Vibhagool, Manoon Leechawengwong, Mondej Sookpranee, Anantasak Apairatana Prevalence of HIV-1 polymerase gene mutations in pre-treated patients in Thailand.. The Southeast Asian journal of tropical medicine and public health. Vol.33, No.1 (2002). Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20523
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Title
Prevalence of HIV-1 polymerase gene mutations in pre-treated patients in Thailand.
Other Contributor(s)
Abstract
To determine the prevalence of drug resistance-conferring mutations in human immunodeficiency virus type 1 (HIV-1), 83 HIV-1 infected Thai patients who had been treated with any antiretroviral drug were studied. HIV-1 RNA was reverse transcribed and amplified by RT-PCR. The direct sequencing of HIV-1 reverse transcriptase (RT) and protease was then performed. Changes in nucleotide and amino acid sequences were determined by comparison with a pNL4-3 reference sequence. Data on mutations associated with resistance to antiretroviral drugs were obtained from literature. The mutations associated with lamivudine resistance (M184V/I) were found most often (in 45.7% of individuals). Zidovudine-resistant mutants: T215Y/F (36%), M41L (28%) and K70R (25.3%) were common; but mutations linked to didanosine (L74V) and multinucleoside-resistant genotypes (Q151M) were rarely recognized (2.4% and 3.6%, respectively). The stavudine-resistant mutant (V75T) and T69 insertions were not found. All subjects who had a significant exposure to antiretroviral drugs and current virological failure in the past carried drug-resistant genotypes. Genotypic resistance to zidovudine, lamivudine, zalcitabine, indinavir and ritonavir appeared in more than one third of the samples, which suggested that the prevalence of the HIV-1 resistance-conferring genotype resisting reverse transcriptase inhibitors and/or protease inhibitors was high in treatment experienced patients.