Publication: Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood
Issued Date
2007-03-01
Resource Type
ISSN
13835769
Other identifier(s)
2-s2.0-33847158906
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology International. Vol.56, No.1 (2007), 65-69
Suggested Citation
Rachanee Udomsangpetch, Sangdao Somsri, Tasanee Panichakul, Kesinee Chotivanich, Jeeraphat Sirichaisinthop, Zhaoqing Yang, Liwang Cui, Jetsumon Sattabongkot Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood. Parasitology International. Vol.56, No.1 (2007), 65-69. doi:10.1016/j.parint.2006.12.005 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/24574
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Title
Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood
Abstract
Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology. © 2006 Elsevier Ireland Ltd. All rights reserved.