Publication: Discovery of opisthorchis lobatus (Trematoda: Opisthorchiidae): A new record of small liver flukes in the greater mekong sub-region
Issued Date
2011-12-01
Resource Type
ISSN
19372345
00223395
00223395
Other identifier(s)
2-s2.0-84855393898
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Parasitology. Vol.97, No.6 (2011), 1152-1158
Suggested Citation
Urusa Thaenkham, Supaporn Nuamtanong, Youthanavanh Vonghachack, Tippayarat Yoonuan, Surapol Sanguankiat, Paron Dekumyoy, Bounlay Prommasack, Jun Kobayashi, Jitra Waikagul Discovery of opisthorchis lobatus (Trematoda: Opisthorchiidae): A new record of small liver flukes in the greater mekong sub-region. Journal of Parasitology. Vol.97, No.6 (2011), 1152-1158. doi:10.1645/GE-2764.1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11230
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Title
Discovery of opisthorchis lobatus (Trematoda: Opisthorchiidae): A new record of small liver flukes in the greater mekong sub-region
Abstract
Metacercariae, morphologically similar to those of small liver flukes, were found to parasitize red-tailed snakehead fish, Channa limbata, collected from the city of Vientiane, Lao People's Democratic Republic. Adult worms that were recovered from experimentally-infected hamsters showed characteristics distinctly different from Opisthorchis viverrini, but closely similar to Opisthorchis lobatus, which was first reported in poultry (Anas sp.) from Pakistan. The present study aimed to redescribe O. lobatus based on the adult worms recovered from experimentally-infected hamsters. Additionally, it aimed to document the genetic relationships among O. lobatus and other opisthorchiid liver flukes using the mitochondrial cytochrome c oxidase subunit 1 (COI) gene and the internal transcribed spacer 2 (ITS2) region. DNA alignment of the O. lobatus and O. viverrini COI partial sequences (330 bp) showed 3.03% fixed differences (2.72% of amino acids changed) while the ITS2 region (350 bp) indicated a 0.86% difference for nucleotides. Species boundaries between the 2 parasites were determined by neighbor-joining analysis using the molecular sequence data. The phenogram confirmed that O. lobatus was distinctly different from O. viverrini, representing the first reported instance of O. lobatus in the Greater Mekong Sub-region (GMS) and the first record of C. limbata as the second intermediate host of a small liver fluke. Questions regarding human infection and the extent of the geographic distribution of these species should be investigated further. © 2011 American Society of Parasitologists.