Co-stimulatory molecules on peripheral blood mononuclear cells and tissue infiltrating cells of skin wart and in vitro poke weed mitogen stimulation

Abstract

Background: Skin wart is a lesion caused by human papilloma viruses (HPVs) that can infect both male and female. Objective: Quantify the number of CD28 +, CD86 +, CD152 + and γδ + in peripheral blood mononuclear cells (PBMCs) of subjects with skin wart. Identify CD86 + and γδ + cells in skin wart cryosections. Material and Method: Sixteen subjects with skin warts on face, hand, finger, knee, foot or plantar, both male and female, aged between 19-59 years-old, were recruited from Ramathibodi Hospital, Mahidol University, Bangkok Results: CD86 and CD152, on peripheral blood mononuclear cells (PBMCs) of subjects with skin wart are significantly lower compared to controls. Tissue cryosection staining for CD86 + and γδ + cells showed no difference among subjects with skin wart and control. Proliferative response to poke weed mitogen of subjects with skin wart is significantly lower than control subjects. Conclusion: There was no difference in the number of subjects positive for CD28 and CD86 + cell between normal and skin wart subject, but an increase in skin wart subjects with γδ + cells.