Publication: Intracellular expression of granzymes A, B, K and M in blood lymphocyte subsets of critically ill patients with or without sepsis
Issued Date
2021-08-01
Resource Type
ISSN
13652249
00099104
00099104
Other identifier(s)
2-s2.0-85106702926
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Clinical and Experimental Immunology. Vol.205, No.2 (2021), 222-231
Suggested Citation
M. Isabel García-Laorden, Arie J. Hoogendijk, Maryse A. Wiewel, Lonneke A. van Vught, Marcus J. Schultz, Niels Bovenschen, Alex F. de Vos, Tom van der Poll Intracellular expression of granzymes A, B, K and M in blood lymphocyte subsets of critically ill patients with or without sepsis. Clinical and Experimental Immunology. Vol.205, No.2 (2021), 222-231. doi:10.1111/cei.13601 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/77251
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Intracellular expression of granzymes A, B, K and M in blood lymphocyte subsets of critically ill patients with or without sepsis
Abstract
Sepsis is a complex syndrome related to an infection-induced exaggerated inflammatory response, which is associated with a high mortality. Granzymes (Gzm) are proteases mainly found in cytotoxic lymphocytes that not only have a role in target cell death, but also as mediators of infection and inflammation. In this study we sought to analyse the intracellular expression of GzmA, B, M and K by flow cytometry in diverse blood lymphocyte populations from 22 sepsis patients, 12 non-infected intensive care unit (ICU) patients and 32 healthy controls. Additionally, we measured GzmA and B plasma levels. Both groups of patients presented decreased percentage of natural killer (NK) cells expressing GzmA, B and M relative to healthy controls, while sepsis patients showed an increased proportion of CD8+ T cells expressing GzmB compared to controls. Expression of GzmK remained relatively unaltered between groups. Extracellular levels of GzmB were increased in non-infected ICU patients relative to sepsis patients and healthy controls. Our results show differential alterations in intracellular expression of Gzm in sepsis patients and non-infected critically ill patients compared to healthy individuals depending on the lymphocyte population and on the Gzm.