Publication: Investigation of pten promoter methylation in ameloblastoma
Issued Date
2020-07-01
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ISSN
16986946
16984447
16984447
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2-s2.0-85087563819
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Mahidol University
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SCOPUS
Bibliographic Citation
Medicina Oral Patologia Oral y Cirugia Bucal. Vol.25, No.4 (2020), e481-e487
Suggested Citation
Puangwan Lapthanasupkul, Boworn Klongnoi, Apiwat Mutirangura, Nakarin Kitkumthorn Investigation of pten promoter methylation in ameloblastoma. Medicina Oral Patologia Oral y Cirugia Bucal. Vol.25, No.4 (2020), e481-e487. doi:10.4317/medoral.23482 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/57851
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Title
Investigation of pten promoter methylation in ameloblastoma
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Abstract
© Medicina Oral S. L. C.I.F. B. Background: Phosphatase and tensin homolog (PTEN) acts as a tumor suppressor gene. Inactivation of PTEN has been reported in various types of cancers. PTEN promoter methylation possibly underlies PTEN inactivation, which results in tumorigenesis. The aim of this study was to investigate whether PTEN promoter methylation contributes to PTEN inactivation in ameloblastoma and its associated protein expression. Material and Methods: In total, 20 fresh-frozen ameloblastoma samples were evaluated for PTEN promoter meth-ylation using methylation-specific polymerase chain reaction (MS-PCR). A subset of 10 paraffin-embedded am-eloblastoma samples was examined for PTEN expression through immunohistochemistry. Four primary cultured ameloblastoma cells were investigated for PTEN promoter methylation and PTEN transcriptional expression via reverse transcription PCR. Results: PTEN promoter methylation was detected in 65% (13/20) of the ameloblastoma samples. Of 10 amelo-blastoma samples, 4 exhibited reduced PTEN expression. Of 5 samples with methylated PTEN, 3 (60%) were associated with loss of PTEN expression. However, PTEN expression was detected in 4 (80%) of 5 samples with unmethylated PTEN. In addition, 3 (75%) of 4 primary ameloblastoma cell cultures exhibited an inverse correlation between PTEN promoter methylation and PTEN transcription level. Conclusions: PTEN promoter methylation is found in a number of ameloblastomas but not significantly correlated with loss of PTEN expression. Genetic or epigenetic mechanisms other than PTEN promoter methylation may contribute to PTEN inactivation in ameloblastoma tumor cells.