Publication: Differential expression patterns of proteins involved in epidermal proliferation and differentiation in canine atopic dermatitis
Issued Date
2012-11-20
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ISSN
01256491
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2-s2.0-84869130811
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Mahidol University
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SCOPUS
Bibliographic Citation
Thai Journal of Veterinary Medicine. Vol.42, No.3 (2012), 287-296
Suggested Citation
Sirin Theerawatanasirikul, Achariya Sailasuta, Roongroje Thanawongnuwech, Tossaporn Nakbed, Komkrid Charngkaew, Gunnaporn Suriyaphol Differential expression patterns of proteins involved in epidermal proliferation and differentiation in canine atopic dermatitis. Thai Journal of Veterinary Medicine. Vol.42, No.3 (2012), 287-296. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15227
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Title
Differential expression patterns of proteins involved in epidermal proliferation and differentiation in canine atopic dermatitis
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Abstract
The pathological alterations in skin keratinocyte proliferation and differentiation in canine atopic dermatitis (CAD) were investigated in 20 small breed dogs with CAD (10 of which displayed lesional CAD skin and 10 nonlesional CAD skin) and 11 healthy control animals. Biopsy of lesional CAD skin showed orthokeratotic hyperkeratosis. Immunohistochemistry (IHC) of Ki-67, a cellular marker for proliferation, showed a higher epidermal cell proliferation rate in the CAD dogs (p < 0.05) which positively correlated with Canine Atopic Dermatitis Extent and Severity Index, CADESI-03 (p < 0.01). IHC labeling indicated reduced expression of cornified envelope proteins, involucrin (IVL) and filaggrin (FLG) in both lesional and non-lesional skin (p < 0.05). In contrast, expression of lympho-epithelial Kazal-type inhibitor (LEKTI), which has been reported to inhibit proteases that cleave proFLG into FLG, was increased in lesional skin compared to the normal controls (p < 0.05). The IHC results showed a positive correlation between CADESI-03 and LEKTI expression and a negative correlation between CADESI-03 and IVL expression. In conclusion, the present study demonstrates that epidermal hyperkeratosis in CAD is related to disturbance of both epidermal proliferation and cornified envelope differentiation. This is the first report of a correlation of LEKTI with CAD.