Publication: Characterization of recombinant malarial RecQ DNA helicase
Issued Date
2014-01-01
Resource Type
ISSN
18729428
01666851
01666851
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2-s2.0-84906190012
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular and Biochemical Parasitology. Vol.196, No.1 (2014), 41-44
Suggested Citation
Pattra Suntornthiticharoen, Witsanu Srila, Porntip Chavalitshewinkoon-Petmitr, Paviga Limudomporn, Montarop Yamabhai Characterization of recombinant malarial RecQ DNA helicase. Molecular and Biochemical Parasitology. Vol.196, No.1 (2014), 41-44. doi:10.1016/j.molbiopara.2014.07.013 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33434
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Title
Characterization of recombinant malarial RecQ DNA helicase
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Abstract
RecQ DNA gene of multi-drug resistant Plasmodium falciparum K1 (PfRecQ1) was cloned, and the recombinant C-terminal-decahistidine-tagged PfRecQ1 was expressed in Escherichia coli. The purified enzyme could efficiently unwind partial duplex DNA substrate in a 3′ to 5′ direction. The malarial RecQ1 could not unwind substrates with both 5′ and 3′ overhangs, those with a 5′ overhang, or blunt-ended DNA duplexes. Unwinding of DNA helicase activity was driven by the hydrolysis of ATP. The drug inhibitory effects of six compounds indicated that only doxorubicin and daunorubicin could inhibit the unwinding activity. © 2014 Elsevier B.V.