Publication:
Inheritance, expression, and silencing of a chitinase transgene in rice

dc.contributor.authorS. Chareonpornwattanaen_US
dc.contributor.authorK. V. Tharaen_US
dc.contributor.authorL. Wangen_US
dc.contributor.authorS. Muthukrishnanen_US
dc.contributor.authorS. K. Dattaen_US
dc.contributor.authorW. Panbangreden_US
dc.contributor.otherKansas State Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherInternational Rice Research Instituteen_US
dc.date.accessioned2018-09-07T08:47:45Z
dc.date.available2018-09-07T08:47:45Z
dc.date.issued1999-01-01en_US
dc.description.abstractThe inheritance and expression of a transgene locus consisting of multiple copies of a rice chitinase gene under the control of the CaMV 35S promoter was studied in the T3and T4generations of a transformed line that expressed the chitinase at a high level. All T3progeny of a homozygous T2parent expressed the chitinase constitutively at 3 weeks after germination, but a proportion of the progeny had undetectable levels of chitinase 8 weeks after germination, indicating silencing of the transgene. Transgene silencing was also observed among progeny of a hemizygous parent. However, we did not observe chitinase gene silencing among progeny of another homozygous line that expressed the transgenic chitinase at a five- to tenfold lower level. Thus, expression level, rather than copy number, of the transgene appears to be critical for silencing. Silencing was observed in the leaf, sheath, and root tissues of the plant, indicating that it is not restricted to specific tissues. Silencing was first observed in the youngest leaves and only later in the oldest leaves of the same plant. There was co-silencing of the selectable marker gene, hpt, which is also driven by the CaMV 35S promoter. Unlike the two transgenes (chitinase and marker), the resident homologous chitinase gene with seed-specific expression and two nonhomologous chitinase genes induced in the leaves upon pathogen infection were not silenced. The silent phenotype was inherited in the T4generation plants, while progeny of expressing plants exhibited silencing. The chitinase transgene appeared intact, and no evidence for gross alterations or methylation of CCGG sites was found. The silent phenotype could not be reversed by treatment with 5-azacytidine. Northern blot analysis and nuclear run-on transcription studies indicated that silencing occurred at the transcriptional level. The implications of transgene silencing in genetic engineering of monocot plants for disease resistance are discussed.en_US
dc.identifier.citationTheoretical and Applied Genetics. Vol.98, No.3-4 (1999), 371-378en_US
dc.identifier.doi10.1007/s001220051082en_US
dc.identifier.issn00405752en_US
dc.identifier.other2-s2.0-0032913363en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/25312
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0032913363&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleInheritance, expression, and silencing of a chitinase transgene in riceen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0032913363&origin=inwarden_US

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